Antioxidant Capacity of the Leaf Extract Obtained from Arrabidaea chica Cultivated in Southern Brazil.docVIP

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Antioxidant Capacity of the Leaf Extract Obtained from Arrabidaea chica Cultivated in Southern Brazil.doc

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Antioxidant Capacity of the Leaf Extract Obtained from Arrabidaea chica Cultivated in Southern Brazil

AntioxidantCapacityoftheLeafExtractObtainedfrom ArrabidaeachicaCultivatedinSouthernBrazil JackelineTiemyGuinozaSiraichi1,3,DanieleFernandaFelipe1,LaraZamparSerraBrambilla1 ,Melissa JunqueiraGatto2,Va?niaAparecidaTerra4,AlessandraLourencoCecchini4,LuciaElaineRanieriCortez5, EdsonRodrigues-Filho6,Dio′genesApar?′cioGarciaCortez1* 1PharmaceuticalSciencesPostgraduateProgram,UniversidadeEstadualdeMaringa′, Maringa′, StateofParana′, Brazil,2DepartmentofPharmacy,UniversidadeEstadual deMaringa′,Maringa′,StateofParana′,Brazil,3InstitutoFederaldoParana′,Londrina,StateofParana′,Brazil,4DepartmentofPathologicalSciences,UniversidadeEstadual de Londrina, Londrina, State of Parana′, Brazil, 5Postgraduate Program in Health Promotion, Centro Universita′rio de Maringa′, State of Maringa′, Parana′, Brazil, 6DepartmentofChemistry,UniversidadeFederaldeSa?o Carlos,Sa?o Carlos,StateofSa?o Paulo,Brazil Abstract Arrabidaeachicaleafextracthasbeenusedbypeopleasananti-inflammatoryandastringentagentaswellasaremedyfor intestinalcolic,diarrhea,leucorrhea,anemia,andleukemia.A.chicaisknowntobeagoodproducerofphenolics.Therefore, inthepresentstudy,weinvestigateditsantioxidantactivity.ThephenoliccompositionofA.chicaleaveswasstudiedby liquid chromatography coupled to diode array detection (LC–DAD) and liquid chromatography coupled to electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS), and isoscutellarein, 6-hydroxyluteolin, hispidulin, scutellarein, luteolin,andapigeninwereidentified.TheextractfromleavesofA.chicawastestedforantioxidantactivityusingthe2,2- diphenyl-1-picrylhydrazyl (DPPH) method, b-carotene bleaching test, and total reactive antioxidant potential (TRAP) method. The crude extract quenched DPPH free radicals in a dose-dependent manner, and the IC of the extract was 50 13.51mg/mL.Theb-carotenebleachingtestshowedthattheadditionoftheA.chicaextractindifferentconcentrations(200 and500mg/mL)preventedthebleachingofb-caroteneatdifferentdegrees(51.2%63.38%and94%64.61%,respe