Behavior of Lactobacilli Isolated from Fermented Slurry (ben-saalga) in Gnotobiotic Rats.docVIP

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Behavior of Lactobacilli Isolated from Fermented Slurry (ben-saalga) in Gnotobiotic Rats.doc

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Behavior of Lactobacilli Isolated from Fermented Slurry (ben-saalga) in Gnotobiotic Rats

BehaviorofLactobacilliIsolatedfromFermentedSlurry (ben-saalga)inGnotobioticRats WilliamsTurpin1*,Christe`leHumblot1,Marie-LouiseNoordine2,3,LauraWrzosek2,3,JulieTomas2,3 , CamilleMayeur2,3,ClaireCherbuy2,3,Jean-PierreGuyot1,MurielThomas2,3 1IRD,UMRNUTRIPASS,IRD/Montpellier2/Montpellier1,Montpellier,France,2INRA,UMR1319Micalis,CommensalandProbiotics-HostInteractionsLaboratory,Jouy-en- Josas,France,3AgroParisTech,UMRMicalis,CommensalandProbiotics-HostInteractionsLaboratory,Jouy-en-Josas,France Abstract Mostbacterialstrains,whichhavebeenstudiedsofarfortheirprobioticfunctions,areextensivelyusedbymanufacturersin developedcountries.Inourwork,wesoughttostudyamix(calledBSL)comprisingthreestrainsbelongingtoLactobacillus fermentum, L. paraplantarum and L. salivarius, that were isolated from a traditional African pearl millet based fermented slurry.OurobjectivewastostudythisBSLcocktailingnotobioticrats,toevaluatetheirsurvivalandtheirbehaviorinthe digestive tract conditions. After a single oral inoculation of germfree rats with BSL, the species established stably in the digestive tract with the following hierarchy of abundance: L. salivarius. L. plantarum. L. fermentum. BSL cocktail was metabolicallyactivesinceitproduced50mMlactateanditexpressedgenesinvolvedinbindingmechanisminthecaecum. Furthermore,theglobalmorphologyofthecolonepitheliumwasnotdisturbedbytheBSLcocktail.BSLcocktaildidnot modify mucus content and host mucus-related genes (MUC1, MUC2, MUC3 or resistin-like molecule b). The cocktail of lactobacilli enhanced the proliferating cell nuclear antigen (PCNA) at a level comparable to what was observed in conventional rats. PCNA was involved in proliferation and DNA repair, but the presence of the cocktail did not provoke proliferativeevents(withKi67asindicator),sowesupposeBSLmayhelpgutpreservation.Thisworkisthefirststeptowards theselectionofstrainsthatarederivedfromtraditionalfermentedfoodtoformulatenewprobioticmixture. Citation: Turpin W, Humblot C, Noordine M-L, Wrzos