bHLH003, bHLH013 and bHLH017 Are New Targets of JAZ Repressors Negatively Regulating JA Responses.docVIP

下载本文档

7
0
约5.68万字
约 12页
2017-05-07 发布于上海
举报
版权申诉

bHLH003, bHLH013 and bHLH017 Are New Targets of JAZ Repressors Negatively Regulating JA Responses.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

bHLH003, bHLH013 and bHLH017 Are New Targets of JAZ Repressors Negatively Regulating JA Responses

bHLH003,bHLH013andbHLH017AreNewTargetsof JAZRepressorsNegativelyRegulatingJAResponses SandraFonseca1,PatriciaFerna′ndez-Calvo1,GuillermoM.Ferna′ndez1,MonicaD?′ez-D?′az1, SelenaGimenez-Ibanez1,IreneLo′pez-Vidriero2,MartaGodoy2,GemmaFerna′ndez-Barbero1,JelleVan Leene3,4,GeertDeJaeger3,4,Jose′ ManuelFranco-Zorrilla2,RobertoSolano1* 1DepartamentodeGeneticaMoleculardePlantas,CentroNacionaldeBiotecnolog?′a–ConsejoSuperiordeInvestigacionesCient?′ficas,CampusUniversidadAuto′noma, Madrid, Spain, 2Genomics Unit, Centro Nacional de Biotecnolog?′a–Consejo Superior de Investigaciones Cient?′ficas, Campus Universidad Auto′noma, Madrid, Spain, 3DepartmentofPlantSystemsBiology,VIB,Gent,Belgium,4DepartmentofPlantBiotechnologyandBioinformatics,GhentUniversity,Gent,Belgium Abstract CellreprogramminginresponsetojasmonatesrequiresatightcontroloftranscriptionthatisachievedbytheactivityofJA- relatedtranscriptionfactors(TFs).Amongthem,MYC2,MYC3andMYC4havebeendescribedasactivatorsofJAresponses. HerewecharacterizedthefunctionofbHLH003,bHLH013andbHLH017thatconformaphylogeneticcladecloselyrelated toMYC2,MYC3andMYC4.WefoundthatthesebHLHsformhomo-andheterodimersandalsointeractwithJAZrepressors in vitro and in vivo. Phenotypic analysis of JA-regulated processes, including root and rosette growth, anthocyanin accumulation, chlorophyll loss and resistance to Pseudomonas syringae, on mutants and overexpression lines, suggested thatthesebHLHsarerepressorsofJAresponses.bHLH003,bHLH013andbHLH017aremainlynuclearproteinsandbind DNA with similar specificity to that of MYC2, MYC3 and MYC4, but lack a conserved activation domain, suggesting that repressionisachievedbycompetitionforthesamecis-regulatoryelements.Moreover,expressionofbHLH017isinducedby JAanddependsonMYC2,suggestinganegativefeed-backregulationoftheactivityofpositiveJA-relatedTFs.Ourresults suggestthatthecompetitionbetweenpositiveandnegativeTFsdeterminestheoutputofJA-dependenttranscriptional activation. Citation:FonsecaS,Ferna′ndez-Calvo P,