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Using metagenomic analyses to estimate the consequences of enrichment bias for pathogen detection 英文论文文献
Pettengilletal.BMCResearchNotes2012,5:378
/1756-0500/5/378
SHORT REPORT
OpenAccess
Usingmetagenomicanalysestoestimatethe
consequencesofenrichmentbiasfor
pathogendetection
JamesBPettengill1,EugeneMcAvoy2,JamesRWhite3,MarcAllard1,EricBrown1andAndreaOttesen1*
Abstract
Background:Enrichingenvironmentalsamplestoincreasetheprobabilityofdetectionhasbeenstandardpractice
throughoutthehistoryofmicrobiology.However,byitsverynature,theprocessofenrichmentcreatesabiased
samplethatmayhaveunintendedconsequencesforsurveillanceorresolvingapathogenicoutbreak.Withthe
adventofnext-generationsequencingandmetageno
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