Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation 英文论文文献.docVIP

Faize et al. BMC Biotechnology 2010, 10:53 /1472-6750/10/53 METHODOLOGY ARTICLE Open Access Using quantitative real-time PCR to detect Methodology article chimeras in transgenic tobacco and apricot and to monitor their dissociation Mohamed Faize2, Lydia Faize1 and Lorenzo Burgos*1 Abstract Background: The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transformed tissues and the presence of a mixture of transgenic and non-transgenic ce