Utilizing high-throughput experimentation to enhance specific productivity of an E.coli T7 expression system by phosphate limitation 英文论文文献.docVIP

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Utilizing high-throughput experimentation to enhance specific productivity of an E.coli T7 expression system by phosphate limitation 英文论文文献.doc

Utilizing high-throughput experimentation to enhance specific productivity of an E.coli T7 expression system by phosphate limitation 英文论文文献

Huberetal.BMCBiotechnology2011,11:22 /1472-6750/11/22 RESEARCH ARTICLE OpenAccess Utilizinghigh-throughputexperimentationto enhancespecificproductivityofanE.coliT7 expressionsystembyphosphatelimitation RobertHuber*,SimonRoth,NatalieRahmen,JochenBüchs* Abstract Background:Thespecificproductivityofcultivationprocessescanbeoptimized,amongstothers,byusinggenetic engineeringofstrains,choiceofsuitablehost/vectorsystemsorprocessoptimization(e.g.choosingtheright inductiontime).Afurtherpossibilityistoreducebiomassbuildupinfavorofanenhancedproductformation,e.g. bylimitingsecondarysubstratesinthemediu

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Utilizing high-throughput experimentation to enhance specific productivity of an E.coli T7 expression system by phosphate limitation 英文论文文献.docVIP