Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays 英文论文文献.docVIP

BMC Research Notes BioMedCentral Technical Note Open Access Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays Thomas Mikeska*1 and Alexander Dobrovic1,2 Address: 1Molecular Pathology Research and Development Laboratory, Department of Pathology, Peter MacCallum Cancer Centre, Locked Bag 1, ABeckett Street, Melbourne, Victoria 8006, Australia and 2Department of Pathology, University of Melbourne, Parkville, Victoria 3010, Australia Email: Thomas Mikeska* - Thomas.Mikeska@; Alexander Dobrovic - Alexander.Dobrovi