Validation of reference genes for quantitative RT-qPCR studies of gene expression in Atlantic cod (Gadus morhua l.) during temperature stress 英文论文文献.docVIP
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Validation of reference genes for quantitative RT-qPCR studies of gene expression in Atlantic cod (Gadus morhua l.) during temperature stress 英文论文文献
Aursnesetal.BMCResearchNotes2011,4:104
/1756-0500/4/104
SHORT REPORT
OpenAccess
Validationofreferencegenesforquantitative
RT-qPCRstudiesofgeneexpressioninAtlantic
cod(Gadusmorhual.)duringtemperaturestress
IngridAAursnes1,AnneLRishovd1,HansEKarlsen2andTorGj?en1*
Abstract
Background:Oneimportantphysiologicalresponsetoenvironmentalstressinanimalsischangeingene
expression.ToobtainreliabledatafromgeneexpressionstudiesusingRT-qPCRitisimportanttoevaluateasetof
possiblereferencegenesasnormalizersforexpression.Theexpressionofthesecandidategenesshouldbe
analyzedintherelevanttissuesduringnormalandstres
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