Validation of reference genes for quantitative RT-qPCR studies of gene expression in Atlantic cod (Gadus morhua l.) during temperature stress 英文论文文献.docVIP

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Validation of reference genes for quantitative RT-qPCR studies of gene expression in Atlantic cod (Gadus morhua l.) during temperature stress 英文论文文献.doc

Validation of reference genes for quantitative RT-qPCR studies of gene expression in Atlantic cod (Gadus morhua l.) during temperature stress 英文论文文献

Aursnesetal.BMCResearchNotes2011,4:104 /1756-0500/4/104 SHORT REPORT OpenAccess Validationofreferencegenesforquantitative RT-qPCRstudiesofgeneexpressioninAtlantic cod(Gadusmorhual.)duringtemperaturestress IngridAAursnes1,AnneLRishovd1,HansEKarlsen2andTorGj?en1* Abstract Background:Oneimportantphysiologicalresponsetoenvironmentalstressinanimalsischangeingene expression.ToobtainreliabledatafromgeneexpressionstudiesusingRT-qPCRitisimportanttoevaluateasetof possiblereferencegenesasnormalizersforexpression.Theexpressionofthesecandidategenesshouldbe analyzedintherelevanttissuesduringnormalandstres

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