干细胞基.pptVIP

* 目前研究的成体干细胞 （Adult Stem Cells） Bone Marrow Heamatopoietic Stem Cells( HSC ) Peripheral Blood Stem Cells ( PBSC ) Fetal Liver Stem Cells Umbilical Cord Blood Stem Cells Neural Stem Cells “Studies suggest that stem cells in different adult tissues may be more similar than previously thought and perhaps in some cases have a developmental repertoire close to that of ES cells. ” Science, Volume 288, Number 5471, Issue of 2 Jun 2000, pp. 1660 - 1663 * 造血干细胞 Hematopoietic Stem Cells，HSC， * 造血干细胞作为干细胞研究 与应用的突破口 1. 造血细胞是细胞增殖分化的最佳模型。 2. 造血干／祖细胞及各系血细胞的表面标志较为清 楚，细胞表型特征可进行定量分析，且可分选。 3. 造血干细胞增殖及向各系分化的重要诱导因 子、受体、信号转导、微环境等因素较为清楚。 4. 造血细胞发挥功能可相对游离，不需“生物支架”、 神经、血管、外科移植等复杂的“下游”工艺，因 此便于直接应用于临床。 * Isolation of Primary and Immortalized CD34– Hematopoietic and Mesenchymal Stem Cells from Various Sources Stem Cells, Vol. 18, No. 1, 1-9, January 2000 * Previous work with mice and human had shown that adult bone marrow stem cells could form liver, as well as bone, cartilage, tendon, nerve and other tissues. 1, Neil Theise et al.; Liver from Bone Marrow in Humans; Hepatology 32, 11-16, July, 2000. 2, Malcolm Alison et al.; Cell Differentiation: Hepatocytes from Non-Hepatic Adult Stem Cells; Nature 406, 257, July 20, 2000. 3, Susan Okie, Bone Marrow Stem Cells Offer Hope for Liver Therapies; Study of Transplants Shows a Transformation; Washington Post, June 27, 2000. 4, Dr. David Whitehouse, Stem cells promise liver repair; BBC, July 19, 2000. 5, Neil Theise et al.; Derivation of hepatocytes from bone marrow cells in mice after radiation-induced myeloablation; Hepatology 31, 235-240, January, 2000. 6, Bryon Petersen et al.; Bone marrow as a potential source of hepatic oval cells; Science 284, 1168-1170; May 14, 1999. * 五、人类干细胞研究的应用 1. ES系统已经成为基因功能研究的有效手段 2. ES细胞系建立后，可从最根本上揭示人及 动物发育过程中的决定基因 3. ES细胞可作为评价新药及化学产品的毒性 及效能的检测系统 4. ES细胞有可能成为今后细胞替代疗法和组 织器官移植的最佳来源 * 人类干细