大鼠骨髓巨噬细胞的分离_纯化_培养以及鉴定_李静.pdfVIP

OI :10.13406/j.cnki.cyxb.2003.04.009 — 436 — 2003 28 4 (Journal of Chongqing Medical University 2003.Vol.28 No.4) :0253-3626(2003)04-0436-04 大鼠骨髓巨噬细胞的分离,纯化,培养以及鉴定 李　静, 王亚平 (, 　400016) 【　】目的:, , 。方法: , 60%DMEM , 20%, 20%(v/v)L929, 6 ～ 7 。 、Wright′s 、 ;;; 。结果:, , , -CD 。结论:、、 68 。 【】;;; 【】R32.43　　　　【】A　　　　【】2002-09-24 Methodology of separation, purification, cultivation and identificationof rat bone marrow macrophage LIJing,et al (Department of Histology and Embryology, Collegeof BasicMedical Sciences, Chongqing Medical University ) 【Abstract】　 bjective:To stu y the biological functions of bone marrow macrophage (BMM Υ)an to establish the metho ology of separation, purification, cultivation an i entification of BMM Υ.Methods:Using the techniques of anchorage- epen ent culture of separate rat bone marrow cell (rBMC)in DMEM culture me ia (contain 20% horse serum, 20% (v/v)L929 con itione me ia)in vitro, a lot of purifie anchor cells were obtaine , an these cells were i entifie with specifically biological marker of macrophage, such as 1, morphologicalobservation:invert phase contrast microscopy, light an electron microscopy;2, enzyme cytochemistry:aci phosphatase (ACP),α-acetic aci naphthol esterase (α-ANE);3, phagocytic experiment:phagocytosis of chicken erythrocytes an prepare Chinese ink;4, immunocytochemistry:surface specific antigen of macrophage (CD stain).Results:The cells w ere purifie 68 having functional satisfactory macrophage accor ing to morphological observation, enzyme cytochemistry, phagocytic experiment an immunocytochemistry.Conclusion:This is a simple an easy metho for separation, purification, cultivation an i entification of rat marrow macrophage