电喷雾串联质谱法分析阿托品在大鼠肠内菌中的代谢产物.pdf

28 3 Vol.28　No.3 2007 8 Journal of Chinese Mass S ectrometry Society Aug.2007 1, 2 2 2 2 陈怀侠 ,杜　鹏,韩凤梅, 陈　勇 (1., 　430062;2., 　430062) n :-(LC-ESIIT-MS ) , , 。 , , , 。 , 、。、、, 。 :-;; :O657.63　　:A 　　:1004-2997(2007)03-169-05 Analysis of the Metabolites of Atropine in Rat Intestinal Flora by Electrospray Ionization Tandem Mass Spectrometry 1, 2 2 2 2 CHEN Huai-xia , DU Peng , HAN Feng-mei , CHEN Yong (1.College of Chemistry and Chemical Engineer, Hubei University , Wuhan 430062, China; 2.Hubei Province K ey Lab of Bio-Technology of Traditional Chinese Medicine, H ubei University , Wuhan 430062, China) Abstract:Atro ine and its metabolites in rat intestinal flora w ere identified by liquid chroma- n togra hy and electros ray ionization tandem mass s ectrometry (LC-ESI-MS ).The condi- tions of chromatogra hic and mass s ectrometric w ere o timized using atro ine standard. The chromatogra hic and mass s ectrometric behavior atterns of atro ine w ere summa- rized.A tro ine w as incubated w ith rat intestinal flora in vitro under lack of oxygen.Identi- fication and structural elucidation of the metabolites were erformed by com aring the full n scan M S s ectra of analyzes w ith those of the arent drug and blank sam le.The results reveal that the arent drug and its dehydrolyzed and hydrolyzed metabolites are found in the incubation solution.This method is sensitive, ra id, sim le and suitable for a