_谷氨酰半胱氨酸合成酶_转化生长因子_1在吸烟大鼠肺组织中的表达.pdfVIP

( J ShanxiM edUniv) 2009 2, 40 (2) ∀ 103∀ : 1007- 6611( 2009) 02- 0103- 05 - 1 许建英, 孙丽娜, 庞 敏 (, 030001) : 目的 - (GCS) - 1( TGF 1) mRNA , TGF1 ( COPD)/ 方法 , COPD, 40 1 2 3, 10 GCSh()TGF1mRNA (RTPCR) GCSh TGF1mRNA 结果 3 12 3 GCShTGF1mRNA GCShTGF1(P 0. 01) 12 3 GC Sh TGF 1mRNA( P 0. 05)! 1 3 GCSh mRNA TGF1, GCSh TGF 1 ; 3 GCShmRNA TGF1 结论 , GCSTGF1 mRNA , ; TGF1 COPD / : ; ; - ; 1 : R563 : A Expression ofGCS and TGF1 in pulmonary tissues of smoking rats U Jianying, SUN L ina, PANG M in (D ep t of R esp iration, F irst Clinica lM edical College, Shanx iM ed ical University, Ta iyuan 03000 , Ch ina) Abstract: Obj ective To explore the role ofTGF1 in oxidant/antioxidant mi balance of smoking ratswith chronic obstructive pulmo nary disease( COPD) by investigating the expression of protein andmRNA of glutamylcysteine synthetase(GCS) and TGF1 and their relations. M ethods Rat smoking devicewasmade by self. Forty rats were randomized into 4 groups: nonsmoking group(n = 10), onemonth smoking group(n = 10), wt omonth smoking group( n= 10), threemonth smoking group( n= 10). The changes ofbron chial epithelia andmacrophageswere compared among 4 groups. Immunohistochem istry, cytochem istry stain and hybridizationwere used to detect the expression ofmRNA and protein of GCSh andTGF1 in bronchial epithelia and alveolarmacrophages, respectively. In addition, the mRNA expression of GCSh and TGF1 in pulmonary tissueswere exam ined by RTPCR. R esu lts Comparedw ith nonsmoking group, the protein expression ofGCSh andTGF1 in bronchial epithelia and alveolarmacrophages in onemonth smok ing group, wt omonth smoking group and threemonth smoking group increased significantly(P 0. 01). Compared with nonsmoking group, the mRNA expression ofGCSh andTGF1 in lung tissue and in bronchial epithelia in onemonth smoking group, twomonth smoking group and threemon