不同分离方法获得的冬虫夏草无性型菌株的交配型.PDF

安徽农业大学学报, 2013, 40(5): 819-822 Journal of Anhui Agricultural University 网络出版时间：2013-8-23 9:17:01 [URL] /kcms/detail/34.1162.S0917.019.html 不同分离方法获得的冬虫夏草无性型菌株的交配型 基因 MAT1-2-1 的初步分析 张 磊，肖岩岩，陈 超，李春如* (安徽农业大学微生物防治省重点实验室，合肥 230036) 摘 要：对采用不同分离方法获得的 40 个冬虫夏草（Ophiocordyceps sinensis ）无性型菌株进行交配型基因 MAT1-2-1 的 PCR 特异扩增，并随机取其中 7 个拼接好的 MAT1-2-1 的碱基序列，与 4 个来自 GenBank 数据在内 的共11个样品构建了系统发育树。结果表明，采自青海的7 个供试菌株，连同GenBank 中来自青海的菌株（FJ654176 ） 共同组成了一大类群，而 GenBank 中其他地区的菌株则聚成另外的类群；随机分离得到的 10 个单子囊孢子菌株全 部含有 MAT1-2-1 基因， MAT1-1: MAT1-2 未见按 4:4 进行分离，提示冬虫夏草极有可能是同宗配型。 关键词：冬虫夏草；无性型菌株；MAT1-2-1 基因；单子囊孢子；同宗配型 中图分类号：Q949.32 文献标识码：A 文章编号：1672−352X (2013)05−0819−04 Preliminary analysis of MAT1-2-1 mating type genes of anamorphic isolates from different sources of Ophiocordyceps sinensis ZHANG Lei, XIAO Yan-yan, CHEN Chao, LI Chun-ru (Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036) Abstract: There is close relationship between the mating type gene of Ophiocordyceps sinensis and the arti- ficial introduction of fruiting body. Many scholars focus on the mating type of O.sinensis, and MAT1-2-1 se- quences of 40 isolates of O.sinensis by different methods were amplified with the primers MAT1-2-1 and evolu- tionary phylogenetic analyses were performed with sequences of the MAT1-2-1 gene, of which 7 sequences of MAT1-2-1 came from this experiment randomly and 4 sequences of MAT1-2-1 cited from the GenBank data. The result showed that 7 isolates collected from Qinghai, together with the isolates from Qinghai in GenBank make up a large group with the 96% bootstrap value and the isolates from the other