白介素1受体_mRNA在小鼠子宫蜕膜中的转录.pdfVIP

DOI :10.13327/j .jj lau.2013.01.026 吉林农业大学学报　2013, 35 (1):72 ～75, 82 http ://xuebao.j Journal of Jilin Agricultural University -mail:j lndxb @ 1ⅡmRNA 于永生, 罗晓彤, 金海国 吉林省农业科学院畜牧分院, 公主岭 136100 　:采用实时定量PCR 方法检测白介素1 受体Ⅱ基因在小鼠子宫 膜组织或细胞中的转录。 获取正常 的小鼠子宫 膜组织和人工诱导 膜化组织, 同时利用酶消化分离小鼠子宫内膜基质细胞, 进行体外诱导 膜化, 以未诱导的子宫内膜基质细胞为对照。 结果表明:白介素1 受体Ⅱ在正常子宫 膜组织、人工诱导 膜 化组织、小鼠子宫内膜 膜化基质细胞中表达水平均显著高于对照, 说明白介素1 受体Ⅱ可能参与子宫内膜 膜化过程。 :白介素1 受体Ⅱ;子宫内膜; 膜化 :Q954.4　　　:A　　　:1000-5684(2013)01-0072-04 :/kcms/detail/22.1100.S0902.002.html :于永生, 罗晓彤, 金海国.白介素 1 受体ⅡmRNA 在小鼠子宫 膜中的转录[ ].吉林农业大学学 报, 2013, 35(1):72-75, 82. Transcription of Receptor Type Ⅱ of Interleukin 1 in Endometrial Decid- ua YU Yong-sheng, LUO Xiao-tong, JIN Hai-guo Branch of Animal Husbandry , JilinAcademy of AgriculturalSciences, Gongzhuling 136100, China Abstract:The aim of this study was to detect the transcription of receptor type Ⅱ (IL1R2)of interleukin 1 in endometrial decidua.Normal decidual tissue and artificial decidual tissue of mice were collected, and endometrial stromal cells (ESCs)of mice were obtained from endometrium by enzymatic dissociation, and were induced to decidualize in vitro.The transcription of IL1R2 in those tissues or cells were mea- sured by Real time PCR.Compared to the control, the levels of IL1R2 increased significantly in normal decidual tissue, artificial decidual tissue, and endometrial decidual cellinduced in vitro, indicating that IL1R2 may be involved in endometrial decidualization .The study lays foundation for researches on decid- ualizaiton mechanism Key words:receptor type Ⅱ of interleukin 1;endometrium;decidualization 　　 , 、、RNA [1-3] (Decidu