响应面法优化金银花绿原酸和木犀草苷的提取工艺_杨彬.pdfVIP

网络出版时间：2015-01-22 14:46 网络出版地址：/kcms/detail/11.1802.TS1446.034.html 响应面法优化金银花绿原酸和木犀草苷的提取工艺 1 * 杨彬 罗磊 朱文学 康新艳 屈政 （河南科技大学食品与生物工程学院，河南洛阳 471023 ） 摘要：为确定金银花中绿原酸和木犀草苷同时提取的最佳工艺条件，以两者得率为指标，在单因素试验的 基础上，采用响应面法对主要工艺参数进行优化并得到回归模型。结果表明：回归模型能较好地反映指标 与工艺参数之间的关系；最佳工艺条件为提取时间1.25h，温度71℃，乙醇浓度60% （V/V ），料液比1:25 （g/mL ），此条件下绿原酸得率为3.741%，木犀草苷得率为0.067% ，与模型预测值相近。 关键词：金银花；绿原酸；木犀草苷；响应面优化 Optimization of extraction technology of chlorogenic acid and cynaroside in honeysuckle by response surface methodology * Yang Bin, Luo Lei , Zhu Wen-xue, Kang Xin-yan, Qu Zheng (College of food and biological engineering, Henan University of Science and Technology, Luoyang 471023) Abstract ：In order to determine the optimum extracting condition of chlorogenic acid and cynaroside from honeysuckle, response surface methodology was employed to optimize primary parameters and establish a regression mode based on the single factor experiment using their yields as indicators. Results show ed that the regression mode properly reflected relationships among indicators and parameters; the optimum extraction condition was time 1.25h, temperature 71 ℃, alcohol concentration 60% （V/V ）and liquid-to-material 1:25 （g/mL ）. Under the optimum extraction condition, the yield of chlorogenic acid and cynaroside were 3.741% and 0.067%