类风湿关节炎外周血单个核细胞和滑膜中人类软骨糖蛋白_39的研究_沈敏.pdfVIP

·928 · 2004 12 43 12 　Chin J Intern Med, December 2004, Vol 43, No.12 ·· -39 沈敏　曾学军　唐福林 　　【】　　-39(HC gp-39)mRNA (RA) (PBMC), RA 。 　31 RA、6 (A)、10 (SpA)、5(SLE)10 , , 7 RA、5 A , (RT)-PCR。 　RA PBMC HC gp-39 mRNA RT-PCR tubulin 0.8690±0.5240, A(P=0.024)、SpA (P=0.049)、SLE(P = 0.043)(P =0.033) 。 A、SpA、SLE。RA HC gp-39 mRNA RT-PCR tubulin 1.9863±1.9397, A (P = 0.04)。 RA A , PBMC HC gp-39 mRNA 。　HC gp-39 mRNA RA PBMC , HC gp-39 RA , T 。 【】　, ;　;　-39 Human cartilage glycoprotein 39 mRNA expressioninperipheral blood and synoviummononuclear cells in ＊ ＊ rheumatoid arthritis　　SHEN Min, ZEN G X ue-j un , TANG Fu-lin.Dep artment of Rheumatism and I mmunolo y, Pekin Union Medical Colle e Hosp ital, Pekin Union Medical Colle e, Chinese Academy of Medical Sciences, Beij in 100730, China Correspondin author :ZEN G X ue-j un, Email:zx uej un @ 【Abstract】　Objective　To investigate the expression of human cartilage glycoprotein 39 (HC gp-39) in peripheral blood mononuclear cells (PBMC )and synovium of rheumatoid arthritis (RA ) patients.Methods　Levels of HC gp-39 mRNA were detected by reverse transcription-polymerase chain reactionin PBMC of 31 patients with RA, 6 with osteoarthritis (A), 10 with spondylarthropathy (SpA), 5 with systemic lupus erythematosus (SLE), and of 10 healthy controls.Levels of HC gp-39 mRNA were also detected in synovium of 7 patients with RA and 5 with A.The expression of HC gp-39 was semi-quantificated by HC gp-39/tubulin ratio.Results　HC gp-39 mRNA expression in PBMC was increased in RA patients (the HC gp-39/tubulin ratio was 0.8690±0.5240), compared with A (P= 0.024), SpA (P=0.049), SLE (P =0.043)and with healthy control subjects (P =0.033).There were no statistically significant differences among A, SpA, SLE and healthy controls.The level of HC gp-39 mRNA express