Analysis of proteomic profiling of mouse embryonic stem cells derived from fertilized, parthenogenetic and androgenetic blastocysts英文文献资料.docVIP

Vol.1, No.1, 1-15 (2011) Stem Cell Discovery doi:10.4236/scd.2011.11001 Analysis of proteomic profiling of mouse embryonic stem cells derived from fertilized, parthenogenetic and androgenetic blastocysts Xiang-Shun Cui , Xing-Hui Shen , Chang-Kwon Lee , Yong-Kook Kang , 1 2 3 4 5 1,* Teruhiko Wakayama , Nam-Hyung Kim 1 2 3 4 5 Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, Korea Department of Histology and Embryology, Harbin Medical University, Harbin, Heilongjiang Province, China Department of Physiology, Konkuk University, Chungju City, Chungbuk, Korea Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea Center for Developmental Biology, RIKEN Kobe, Kobe, Japan; nhkim@chungbuk.ac.kr Received January 23 2011; revised March 13 2011; accepted March 20 2011. ABSTRACT teins, ANXA5, CLIC1 and SRM, Western blot analysis corroborated the expression patterns indicated by the 2-DE results. ANXA5 and CLIC1 were increased in expression and SRM was de- creased in expression in fESCs compared with both pESCs and aESCs. The differentially ex- pressed proteins identified in the present study warrant further investigation towards the goal of their potential application in ESC therapy. Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of preimplantation embryos. ESCs exhibit true pluripotency, i.e., the ability to differentiate into cells of all three germ layers in the developing embryo. We used 2-DE MALDI-TOF/TOF to identify differentially expressed proteins among three types of mouse embryonic stem cells (ESCs) derived from fer- tilized, parthenogenetic, and androgenetic (fESC, pESC and aESC, respectively) blastocysts.