Augmentation of NKT and NK cell-mediated cytotoxicity by peptidoglycan monomer linked with zinc英文文献资料.docVIP

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Augmentation of NKT and NK cell-mediated cytotoxicity by peptidoglycan monomer linked with zinc英文文献资料.doc

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Augmentation of NKT and NK cell-mediated cytotoxicity by peptidoglycan monomer linked with zinc英文文献资料

Research Communication Mediators of Inflammation, 11, 129–135 (2002) B ACKGROUND: Peptidoglycan monomer (PGM), which Augmentation of NKT and NK cell-mediated cytotoxicity by peptidoglycan monomer linked with zinc was originally prepared by biosynthesis from culture fluids of penicillin-treated Brevibacterium divarica- tum, is an immunostimulator, the activities of which might be improved by addition of zinc (Zn) to the basic molecule. Methods: To test the possible cytotoxic effects of this new analogue, we analyzed the ability of PGM-Zn and PGM to change the phenotypic profile of hepatic and splenic mononuclear lymphatic cells and to affect the growthofmalignantT-cellline YAC-1andsyngeneic thymocytes. ? Ines Mrakovc?ic′-Sutic′, Biserka Rados?evic′-Stas?ic′CA Marija Simin, Damir Muhvic′ and Daniel Rukavina , ? Results: Pretreatment of C57BL/6 mice primarily with PGM-Zn over 6 days (10/mg/kg intraperitoneally) Department of Physiology and Immunology, Faculty of Medicine, University of Rijeka, B. Branchetta 22, HR-51000 Rijeka, Croatia significantly enhanced the proportions of NK1.1high+ , CD4 – CD8 – , CD69 + , and CD3 intermediate /NK1.1 + /IL2R-b (NKT) cells in the liver, and major histocompatibility complex class II cells in the spleen. + + , CD69 , and CD8 + + Both types of cells were highly cytotoxic against YAC- 1 and syngeneic thymocytes, increasing the destruc- tion of YAC-1 by 70% on addition of hepatic cells and by 30% on addition of splenic cells. Destruction of thymocytes increased by 10 and 50%, respectively. Conclusion: The results point to PGM-Zn as a potent cytotoxicity-inducing agent, which also generates autoreactive NKT cells. CA Corresponding Author Tel: +385 51 651 150 Fax: +385 51 675 699 E-mail: biserr@medri.hr Key words: NK cells, NK1.1, Intermediate T cell receptor expressing cells, Cytotoxicity to YAC-1 and syngeneic thymocytes, Liver and spleen, Self-reacting NKT cells Introduction both unspeci