口服甘草次酸对大鼠血清中六种微量元素的影响.doc

口服甘草次酸对大鼠血清中六种金属元素的影响 曹姗1,2，祖元刚1,2(，张琳1,2 （1.东北林业大学森林植物生态学教育部重点实验室，黑龙江，哈尔滨 150040；2.东北林业大学林业生物制剂教育部工程研究中心，黑龙江，哈尔滨 150040）) 浓度，并分析甘草次酸对血清中6种元素的影响。检测结果表明大鼠口服甘草次酸后血清中Na、Cu元素浓度与甘草次酸血药浓度变化趋势相同。血药浓度在给药后2 h达峰值，血清中Na、Cu元素在给药后4 h浓度有显著增加（P 0.05）。随着给药剂量的增大，血清中Na、Cu元素浓度升高，当给药剂量为400 mg·kg-1和200 mg·kg-1 时Na、Cu元素与对照组相比有显著性升高（P 0.05），对其它元素浓度则无显著性影响。本实验研究探讨了口服甘草次酸引起大鼠血清中六种金属元素的变化，为甘草次酸在临床中使用的副作用提供了实验依据。 [关键词] 甘草次酸; LC-MS/MS; 火焰原子吸收光谱法; 金属元素 Effect of glycyrrhetinic acid on metal elements in serum of rats [Abstract] To determine glycyrrhetinic acid concentration in plasma and explore the effect of glycyrrhetinic acid in an oral administration mode on calcium (Ca), copper (Cu), iron (Fe), potassium (K), magnesium (Mg) and sodium (Na) in serum of rats.The concentration of glycyrrhetinic acid was detected in plasma by LC-MS/MS method, and the contents of six elements were determined in serum by flame atomic absorption spectrometry at the conditions of different time periods and administration dosages. A similar change trend between between the concentration of glycyrrhetinic acid in plasma and contents of Na, Cu elements in serum after oral administration of glycyrrhetinic acid was observed. Glycyrrhetinic acid in plasma after administration for 2 h reached the highest level. Meanwhile, the contents of Na and Cu at the administration of glycyrrhetinic acid for 4 h exhibited a significant increase (P 0.05). Moreover, the increased glycyrrhetinic acid dosage could result in the accumulation of Cu and Na in serum of rats. Compared with the control group, the contents of Cu and Na in the groups orally administered with glycyrrhetinic acid at the dosages of 200 and 400 mg/kg revealed a significant increase (P 0.05). However, glycyrrhetinic acid did not exhibit the great impact on the content of other elements in serum. Glycyrrhetinic acid at various oral administration dosages can induce the content changes of Ca, Cu, Fe, K, Mg and Na in serum. This investigation provides a theoretical