原创力文档Rapid Site-Directed Mutagenesis Using Two-PCR-Generated DNA Fragments Reproducing the Plasmid Template 外文参考文献.docVIP
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Rapid Site-Directed Mutagenesis Using Two-PCR-Generated DNA Fragments Reproducing the Plasmid Template 外文参考文献
JournalofBiomedicineandBiotechnology?2003:3(2003)202–207?PII.S1110724303209141?
RESEARCHARTICLE
RapidSite-DirectedMutagenesisUsing
Two-PCR-GeneratedDNAFragmentsReproducing
thePlasmidTemplate
FloreAllemandou,J¨urgNussberger,HansR.Brunner,andNoureddineBrakch
?
DivisionofHypertensionandVascularMedicine,UniversityHospital,CH-1011Lausanne,Switzerland
Received 10September2002;accepted29October 2002
Wedescribeanewrapidande?cientpolymerasechainreaction(PCR)-basedsite-directedmutagenesismethod.Thisprocedure
ise?ectivewithanyplasmidanditemploysfouroligonucleotideprimers.Oneprimercontainsthedesiredmu
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