EcolisystemsandrecombinationDeterminantsofdivers大肠杆菌系统和潜水员重组的影响因素.pptVIP

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EcolisystemsandrecombinationDeterminantsofdivers大肠杆菌系统和潜水员重组的影响因素.ppt

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EcolisystemsandrecombinationDeterminantsofdivers大肠杆菌系统和潜水员重组的影响因素

E.coli systems and recombination: Determinants of diversity: Overall aims ML Nine/ten lectures with Key topics. Homologous recombination and DNA repair Role of methylation and repair. Role of Plasmids; control of replication, transfer and stability. Illegitimate recombination: transposons and integrons Regulation of DNA transposition. You should: Have a basic grounding for further reading and other systems covered in the course (e.g pathogens). Be able to critically read key papers in the area. Critically assess the development of ideas to date. DNA Repair mechanisms This section will review: The Role of DNA damage and its repair in the generation of genetic diversity in bacteria The mechanistic links of repair with recombination systems covered earlier You should be able to discuss the effect of environment on damage and repair. For example: Chemical and Radiation effects Phagocytic damage in relation to pathogen survival in the host Errors in replication and their repair. How can DNA become damaged? Mismatched bases Polymerase error rate about 1 in 104 (see later lectures) Deamination of C to U leading to mismatch Missing bases. Hydrolysis of purine-deoxyribose bond leading to AP-site. Structural damage. Dimer formation. Broken phosphodiester bonds. Chemicals/radiation REPAIR MECHANISMS NECESSARY FOR SURVIVAL. Types of DNA Damage Summarised General repair mechanisms needed EITHER Reverse damage (e.g. PHOTOREACTIVATION) OR excise DNA and patch repair the region General repair mechanisms needed: Excision repair. Discovered first as a general mechanism in 1964 T- Phage HOST CELL REACTIVATION Excision repair........ Other repair routes. How does UV light cause mutations? Discovery of error-prone repair. SOS System in E.coli SOS System in E.coli Post Replication / Recombination Repair Post Replication / Recombination Repair M J Larkin Biology Biochemistry. The Queen’s University of Belfast. G A C T ds DNA Break Mismatch Thymidine dimer AP site Covalent