A Key Role for Chd1 in Histone H3 Dynamics at the 3′ Ends of Long Genes in Yeast 英文参考文献.docVIP

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A Key Role for Chd1 in Histone H3 Dynamics at the 3′ Ends of Long Genes in Yeast 英文参考文献.doc

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A Key Role for Chd1 in Histone H3 Dynamics at the 3′ Ends of Long Genes in Yeast 英文参考文献

AKeyRoleforChd1inHistoneH3Dynamicsatthe39 EndsofLongGenesinYeast MartaRadman-Livaja1.,TiffaniK.Quan2.,LourdesValenzuela2,JenniferA.Armstrong3,Tiborvan Welsem4,TaeSooKim5,LauraJ.Lee3,StephenBuratowski5,FredvanLeeuwen4,OliverJ.Rando1*, GrantA.Hartzog2* 1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America, 2DepartmentofMolecular,Cell,andDevelopmentalBiology,UniversityofCaliforniaSantaCruz,SantaCruz,California,UnitedStatesofAmerica,3W.M.KeckScience Department,Scripps,ClaremontMcKenna,andPitzerColleges,Claremont,California,UnitedStatesofAmerica,4DivisionofGeneRegulation,NetherlandsCancerInstitute and Netherlands Proteomics Centre, Amsterdam, The Netherlands, 5Department of Biological Chemistry and Molecular Pharmacology, Harvard University, Boston, Massachusetts,UnitedStatesofAmerica Abstract Chd proteins are ATP–dependent chromatin remodeling enzymes implicated in biological functions from transcriptional elongation to control of pluripotency. Previous studies of the Chd1 subclass of these proteins have implicated them in diverse roles in gene expression including functions during initiation, elongation, and termination. Furthermore, some evidencehassuggestedaroleforChd1inreplication-independenthistoneexchangeorassembly.Here,weexamineroles ofChd1inreplication-independentdynamicsofhistoneH3inbothDrosophilaandyeast.Wefindevidenceofarolefor Chd1inH3dynamicsinbothorganisms.Usinggenome-wideChIP-on-chipanalysis,wefindthatChd1influenceshistone turnoveratthe59and39endsofgenes,acceleratingH3replacementatthe59endsofgeneswhileprotectingthe39endsof genesfromexcessiveH3turnover.AlthoughconsistentwithadirectroleforChd1inexchange,theseresultsmayindicate thatChd1stabilizesnucleosomesperturbedbytranscription.Curiously,weobserveastrongeffectofgenelengthonChd1’s effects on H3 turnover. Finally, we show that Chd1 also affects histone modification patterns over genes, likely as a consequence