- 1、本文档共15页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
A Multi-Omics Analysis of Recombinant Protein Production in Hek293 Cells 英文参考文献
AMulti-OmicsAnalysisofRecombinantProtein
ProductioninHek293Cells
StefanieDietmair*,MarkP.Hodson,Lake-EeQuek,NicholasE.Timmins,PeterGray,LarsK.Nielsen
AustralianInstituteforBioengineeringandNanotechnology(AIBN),TheUniversityofQueensland,Brisbane,Queensland,Australia
Abstract
Hek293cellsarethepredominanthostsfortransientexpressionofrecombinantproteinsandareusedforstableexpression
of proteins where post-translational modifications performed by CHO cells are inadequate. Nevertheless, there is little
informationavailableonthekeycellularfeaturesunderpinningrecombinantproteinproductioninHek293cells.Toimprove
our understanding of recombinant protein production in Hek293 cells and identify targets for the engineering of an
improvedhostcellline,wehavecomparedastable,recombinantproteinproducingHek293celllineanditsparentalcell
lineusingacombinationoftranscriptomics,metabolomicsandfluxomics.Producerculturesconsumedlessglucosethan
non-producer cultures while achieving the same growth rate, despite the additional burden of recombinant protein
production.Surprisingly,therewasnoindicationthatproducerculturescompensatedforthereductioninglycolyticenergy
byincreasingtheefficiencyofglucoseutilizationorincreasingglutamineconsumption.Incontrast,glutamineconsumption
waslowerandthemajorityofgenesinvolvedinoxidativephosphorylationweredownregulatedinproducercultures.We
observedanoveralldownregulationofalargenumberofgenesassociatedwithbroadcellularfunctions(e.g.,cellgrowth
andproliferation)inproducercultures,andthereforespeculatethatabroadadaptationofthecellularnetworkfreedup
resources for recombinant protein production while maintaining the same growth rate. Increased abundance of genes
associatedwithendoplasmicreticulumstressindicatedapossiblebottleneckatthepointofproteinfoldingandassembly.
Citation:DietmairS,HodsonMP,QuekL-E,TimminsNE,GrayP,etal.(2012)AMulti-OmicsAnalysisofRecombinantProteinProductioninHek293Cells.PLoS
ONE7(8):e43394.doi:10.1371/journal.pone.0043394
Editor:MikaelR?rdamAnd
您可能关注的文档
- A Look Inside HIV Resistance through Retroviral Protease Interaction Maps 英文参考文献.doc
- A Low Dose of Fermented Soy Germ Alleviates Gut Barrier Injury, Hyperalgesia and Faecal Protease Activity in a Rat Model of Inflammatory Bowel Disease 英文参考文献.doc
- A Low-Tech Analytical Method for Diethylcarbamazine Citrate in Medicated Salt 英文参考文献.doc
- A Loud Auditory Stimulus Overcomes Voluntary Movement Limitation in Cervical Dystonia 英文参考文献.doc
- A Macaque Model of SARS 英文参考文献.doc
- A Low Dimensional Description of Globally Coupled Heterogeneous Neural Networks of Excitatory and Inhibitory Neurons 英文参考文献.doc
- A Macaque's-Eye View of Human Insertions and Deletions Differences in Mechanisms 英文参考文献.doc
- A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand-Specific Multiplex RNA-Seq 英文参考文献.doc
- A Luminal Glycoprotein Drives Dose-Dependent Diameter Expansion of the Drosophila melanogaster Hindgut Tube 英文参考文献.doc
- A Machine Learning Approach for Identifying Novel Cell Type–Specific Transcriptional Regulators of Myogenesis 英文参考文献.doc
文档评论(0)