A New Cell-Selective Three-Dimensional Microincubator Based on Silicon Photonic Crystals 英文参考文献.docVIP

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A New Cell-Selective Three-Dimensional Microincubator Based on Silicon Photonic Crystals 英文参考文献.doc

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A New Cell-Selective Three-Dimensional Microincubator Based on Silicon Photonic Crystals 英文参考文献

ANewCell-SelectiveThree-DimensionalMicroincubator BasedonSiliconPhotonicCrystals FrancescaCarpignano1,GloriaSilva1,SalvatoreSurdo2,ValentinaLeva3,AlessandraMontecucco3, FrancescaAredia3,AnnaIvanaScovassi3,SabinaMerlo1*,GiuseppeBarillaro2,GiulianoMazzini3,4 1Dipartimento di Ingegneria Industriale e dell’Informazione, Universita` di Pavia, Pavia, Italy, 2Dipartimento di Ingegneria dell’Informazione, Elettronica, Informatica, Telecomunicazioni,Universita` diPisa,Pisa,Italy,3IGM-CNR,Pavia,Italy,4DipartimentodiBiologiaeBiotecnologie‘‘L.Spallanzani’’,Universita` diPavia,Pavia,Italy Abstract Inthiswork,weshowthatvertical,highaspect-ratio(HAR)photoniccrystals(PhCs),consistingofperiodicarraysof5mm widegapswithdepthof50mmseparatedby3mmthicksiliconwalls,fabricatedbyelectrochemicalmicromachining,canbe usedasthree-dimensionalmicroincubators,allowingcelllinestobeselectivelygrownintothegaps.Siliconmicromachined dice incorporating regions with different surface profiles, namely flat silicon and deeply etched PhC, were used as microincubators for culturing adherent cell lines with different morphology and adhesion properties. We extensively investigatedandcomparedtheproliferativebehavioronHARPhCsofeighthumancellmodels,withdifferentorigins,such astheepithelial(SW613-B3;HeLa;SW480;HCT116;HT29)andthemesenchymal(MRC-5V1;CF;HT1080).Wealsoverifiedthe contributionofcellsedimentationintothesilicongaps.Fluorescencemicroscopyanalysishighlightsthatonlycelllinesthat exhibit,inthetestedculturecondition,thebehaviortypicalofthemesenchymalphenotypeareabletopenetrateintothe gapsofthePhC,extendingtheirbodydeeplyinthenarrowgapsbetweenadjacentsiliconwalls,andtogrowadherentto the vertical surfaces of silicon. Results reported in this work, confirmed in various experiments, strongly support our statement that such three-dimensional microstructures have selection capabilities with regard to the cell lines that can actively populate the narrow gaps. Cells with a mesenchymal phenotype could be exp