A Nuclear Localization of the Infectious Haematopoietic Necrosis Virus NV Protein Is Necessary for Optimal Viral Growth 英文参考文献.docVIP

ANuclearLocalizationoftheInfectiousHaematopoietic NecrosisVirusNVProteinIsNecessaryforOptimalViral Growth MyeongKyuChoi1,5.,ChangHoonMoon1,7.,MyoungSeokKo1.,Unn-HwaLee1,WhaJaCho2,SeungJu Cha2,JeongWanDo3,GangJoonHeo4,SooGeunJeong5,YooSikHahm5,AbdallahHarmache6 ,Michel Bremont6,GaelKurath7,JeongWooPark1* 1 Department of Biological Sciences, University of Ulsan, Ulsan, Korea,2 Biomedical Research Center, Ulsan University Hospital, College of Medicine, University of Ulsan, Ulsan, Korea,3 South and West Sea Fisheries Research Institute, National Fisheries Research and Development Institute, Yeosu, Korea,4 College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea,5 Ulsan Institute of Health and Environment, Ulsan, Korea,6 Unite de Virologie and Immunologie Moleculaires, INRA CRJ, Domaine de Vilvert, Jouy en Josas, France,7 US Geological Survey, Western Fisheries Research Center, Seattle, Washington, United States of America Abstract The nonvirion (NV) protein of infectious hematopoietic necrosis virus (IHNV) has been previously reported to be essential for efficient growth and pathogenicity of IHNV. However, little is known about the mechanism by which the NV supports the viral growth. In this study, cellular localization of NV and its role in IHNV growth in host cells was investigated. Through transient transfection in RTG-2 cells of NV fused to green fluorescent protein (GFP), a nuclear localization of NV was demonstrated. Deletion analyses showed that the 32EGDL35 residues were essential for nuclear localization of NV protein, and fusion of these 4 amino acids to GFP directed its transport to the nucleus. We generated a recombinant IHNV, rIHNV- NV-DEGDL in which the 32EGDL35 was deleted from the NV. rIHNVs with wild-type NV (rIHNV-NV) or with the NV gene replaced with GFP (rIHNV-DNV-GFP) were used as controls. RTG-2 cells