A Perikinetochoric Ring Defined by MCAK and Aurora-B as a Novel Centromere Domain 英文参考文献.docVIP

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2017-05-11 发布于上海
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A Perikinetochoric Ring Defined by MCAK and Aurora-B as a Novel Centromere Domain 英文参考文献.doc

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A Perikinetochoric Ring Defined by MCAK and Aurora-B as a Novel Centromere Domain 英文参考文献

APerikinetochoricRingDefinedbyMCAK andAurora-BasaNovelCentromereDomain Mar?′aTeresa Parra1*,Roc?′oGo′mez1,Alberto Viera1,Jesu′sPage1,Adela Calvente1,Linda Wordeman2,Julio S.Rufas1, Jose′ A.Suja1 1DepartamentodeBiolog?′a,UniversidadAuto′nomadeMadrid,Madrid,Spain2DepartmentofPhysiologyandBiophysics,SchoolofMedicine,UniversityofWashington, Seattle,Washington,UnitedStatesofAmerica MitoticCentromere-AssociatedKinesin(MCAK)isamemberofthekinesin-13subfamilyofkinesin-relatedproteins.In mitosis, this microtubule-depolymerising kinesin seems to be implicated in chromosome segregation and in the correction of improper kinetochore-microtubule interactions, and its activity is regulated by the Aurora-B kinase. However,therearenopublisheddataonitsbehaviourandfunctionduringmammalianmeiosis.Wehaveanalysedby immunofluorescenceinsquashedmousespermatocytes,thedistributionandpossiblefunctionofMCAK,togetherwith Aurora-B, during both meiotic divisions. Our results demonstrate that MCAK and Aurora-B colocalise at the inner domain of metaphase I centromeres. Thus, MCAK shows a ‘‘cone’’-like three-dimensional distribution beneath and surroundingthecloselyassociatedsisterkinetochores.Duringthesecondmeioticdivision,MCAKandAurora-Balso colocaliseattheinnercentromeredomainasabandthatjoinssisterkinetochores,butonlyduringprometaphaseIIin unattachedchromosomes.DuringchromosomecongressiontothemetaphaseIIplate,MCAKrelocalisesandappears as a ring below each sister kinetochore. Aurora-B also relocalises to appear as a ring surrounding and beneath kinetochoresbutduringlatemetaphaseII.OurresultsdemonstratethattheredistributionofMCAKatprometaphase II/metaphaseIIcentromeresdependsontensionacrossthecentromereand/orontheinteractionofmicrotubuleswith kinetochores.WeproposethattheperikinetochoricringsofMCAKandAurora-Bdefineanoveltransientcentromere domain at least in mouse chromosomes during meiosis. We discuss the possible functions of MCAK at the inner centromeredomainandattheperikinetochoricringduring