A Peptide That Binds Specifically to the β-Amyloid of Alzheimers Disease Selection and Assessment of Anti-β-Amyloid Neurotoxic Effects 英文参考文献.docVIP

下载本文档

7
0
约 9页
2017-05-11 发布于上海
举报
版权申诉

A Peptide That Binds Specifically to the β-Amyloid of Alzheimers Disease Selection and Assessment of Anti-β-Amyloid Neurotoxic Effects 英文参考文献.doc

1、本文档共9页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

APeptideThatBindsSpecificallytotheb-Amyloidof Alzheimer’sDisease:SelectionandAssessmentof Anti-b-AmyloidNeurotoxicEffects FangWang1,Xian-LingZhou1,Qi-GangYang1,Wen-HuaXu1,FeiWang1,Yong-PingChen2, Gui-HaiChen1* 1Department of Neurology, The First Affiliated Hospital of Anhui Medical University, Hefei, People’s Republic of China, 2Department of Biomedical Engineering, JohnsHopkinsUniversity,Baltimore,Maryland,UnitedStatesofAmerica Abstract The accumulation of the amyloid-b peptide (Ab) into amyloid plaques, an essential event in Alzheimer’s disease (AD) pathogenesis,hascausedresearcherstoseekcompoundsthatphysiologicallybindAbandmodulateitsaggregationand neurotoxicity.InordertodevelopnewAb-specificpeptidesforAD,arandomized12-merpeptidelibrarywithAb1-10asthe targetwasusedtoidentifypeptidesinthepresentstudy.Afterthreeroundsofselection,specificphageswerescreened, andtheirbindingaffinitiestoAb1-10werefoundtobehighlyspecific.Finally,aspecialpeptidewassynthesizedaccordingto thesequencesoftheselectedphages.Inaddition,theeffectsofthespecialpeptideonAbaggregationandAb-mediated neurotoxicityinvitroandinvivowereassessed.Theresultsshowthatthespecialpeptidenotonlyinhibitedtheaggregation of Ab into plaques, but it also alleviated Ab-induced PC12 cell viability and apoptosis at appropriate concentrations as assessed by the cell counting kit-8 assay and propidium iodide staining. Moreover, the special peptide exhibited a protectiveeffectagainstAb-inducedlearningandmemorydeficitsinrats,asdeterminedbytheMorriswatermazetask.In conclusion,weselectedapeptidethatspecificallybindsAb1-10andcanmodulateAbaggregationandAb-inducedneuronal damage.ThisopensuppossibilitiesforthedevelopmentofanoveltherapeuticapproachforthetreatmentofAD. Citation:WangF,ZhouX-L,YangQ-G,XuW-H,WangF,etal.(2011)APeptideThatBindsSpecificallytotheb-AmyloidofAlzheimer’sDisease:Selectionand AssessmentofAnti-b-AmyloidNeurotoxicEffects.PLoSONE6(11):e27649.doi:10.1371/journal.pone.0027649 Editor:MaximAntopolsky,UniversityofHelsi