A Piezoelectric Immunosensor Using Hybrid Self-Assembled Monolayers for Detection of Schistosoma japonicum 英文参考文献.docVIP

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A Piezoelectric Immunosensor Using Hybrid Self-Assembled Monolayers for Detection of Schistosoma japonicum 英文参考文献.doc

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A Piezoelectric Immunosensor Using Hybrid Self-Assembled Monolayers for Detection of Schistosoma japonicum 英文参考文献

APiezoelectricImmunosensorUsingHybridSelf- AssembledMonolayersforDetectionofSchistosoma japonicum ShipingWang1*,TieqiuYin1,2,ShaohuaZeng1,HongliChe1,FeifeiYang1,XiuchunChen1,GuoliShen3, ZhaoyangWu3 1KeyLaboratoryofSchistosomiasisinHunan,DepartmentofParasitology,XiangyaMedicalCollege,CentralSouthUniversity,Changsha,China,2TheSecondPeople’s HospitalofHunanProvinceandBrainsHospitalofHunanProvince,DepartmentofClinicalLaboratory,Changsha,China,3StateKeyLaboratoryofChemo/Biosensingand Chemometrics,CollegeofChemistryandChemicalEngineering,HunanUniversity,Changsha,China Abstract Background:TheparasiteSchistosomajaponicumcausesschistosomiasisdisease,whichthreatenshumanlifeandhampers economic and social development in some Asian countries. An important lesson learned from efforts to reduce the occurrence of schistosomiasis is that the diagnostic approach must be altered as further progress is made towards the controlandultimateeliminationofthedisease. Methodology/PrincipalFindings:Usingmixedself-assembledmonolayermembrane(mixedSAM)technology,amixtureof mercaptopropionic acid (MPA) and mercaptoethanol (ME) was self-assembled on the surface of quartz crystals by gold- sulphur-bonds. Soluble egg antigens (SEA) of S. japonicum were then cross-linked to the quartz crystal using a special coupling agent. As compared withthetraditional single self-assembled monolayer immobilization method, S.japonicum antigen (SjAg) immobilization using mixed self-assembled monolayers exhibits much greater immunoreactivity. Under optimalexperimentalconditions,thedetectionrangeis1:1500to1:60(infectedrabbitserumdilutionratios).Wemeasured severalinfectedrabbitserumsampleswithvaryingS.japonicumantibody(SjAb)concentrationsusingbothimmunosensor andELISAtechniquesandthenproducedacorrelationanalysis.Thecorrelationcoefficientsreached0.973. Conclusions/Significance: We have developed a new, simple, sensitive, and reusable piezoelectric immunosensor that directly detects SjAb in the serum. This method may re