A Water Soluble CoQ10 Formulation Improves Intracellular Distribution and Promotes Mitochondrial Respiration in Cultured Cells 英文参考文献.docVIP

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A Water Soluble CoQ10 Formulation Improves Intracellular Distribution and Promotes Mitochondrial Respiration in Cultured Cells 英文参考文献.doc

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A Water Soluble CoQ10 Formulation Improves Intracellular Distribution and Promotes Mitochondrial Respiration in Cultured Cells 英文参考文献

AWaterSolubleCoQ10 FormulationImproves IntracellularDistributionandPromotesMitochondrial RespirationinCulturedCells ChristianBergamini1,NoahMoruzzi1,AntonellaSblendido2,GiorgioLenaz1,RomanaFato1* 1DepartmentofBiochemistry‘‘G.Moruzzi’’,UniversityofBologna,Bologna,Italy,2ScharperTherapeutics,MedicalDepartment,SestoS.Giovanni,Milano,Italy Abstract Background:Mitochondriaareboththecellularpowerhouseandthemajorsourceofreactiveoxygenspecies.Coenzyme Q10playsakeyroleinmitochondrialenergyproductionandisrecognizedasapowerfulantioxidant.Forthesereasonsit can be argued that higher mitochondrial ubiquinone levels may enhance the energy state and protect from oxidative stress.DespitethelargenumberofclinicalstudiesontheeffectofCoQ10supplementation,thereareveryfewexperimen- tal data about the mitochondrial ubiquinone content and the cellular bioenergetic state after supplementation. Controversialclinicalandinvitroresultsaremainlyduetothehighhydrophobicityofthiscompound,whichreducesits bioavailability. PrincipalFindings:Wemeasuredthecellularandmitochondrialubiquinonecontentintwocelllines(T67andH9c2)after supplementationwithahydrophilicCoQ10formulation(QterH)andnativeCoQ10.Ourresultsshowthatthewatersoluble formulation is more efficient in increasing ubiquinone levels. We have evaluated the bioenergetics effect of ubiquinone treatment, demonstrating that intracellular CoQ10 content after Qter supplementation positively correlates with an improvedmitochondrialfunctionality(increasedoxygenconsumptionrate,transmembranepotential,ATPsynthesis)and resistancetooxidativestress. Conclusions:TheimprovedcellularenergymetabolismrelatedtoincreasedCoQ10contentrepresentsastrongrationalefor theclinicaluseofcoenzymeQ10andhighlightsthebiologicaleffectsofQterH,thatmakeittheeligibleCoQ10 fortheubiquinonesupplementation. formulation Citation: Bergamini C, MoruzziN, SblendidoA, Lenaz G, Fato R(2012) AWater SolubleCoQ10 FormulationImproves Intracellular Distribution and Promotes MitochondrialRespiratio