A Yeast Two-Hybrid Screen for SYP-3 Interactors Identifies SYP-4, a Component Required for Synaptonemal Complex Assembly and Chiasma Formation in Caenorhabditis elegans Meiosis 英文参考文献.docVIP

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A Yeast Two-Hybrid Screen for SYP-3 Interactors Identifies SYP-4, a Component Required for Synaptonemal Complex Assembly and Chiasma Formation in Caenorhabditis elegans Meiosis 英文参考文献.doc

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A Yeast Two-Hybrid Screen for SYP-3 Interactors Identifies SYP-4, a Component Required for Synaptonemal Complex Assembly and Chiasma Formation in Caenorhabditis elegans Meiosis 英文参考文献

AYeastTwo-HybridScreenforSYP-3Interactors IdentifiesSYP-4,aComponentRequiredfor SynaptonemalComplexAssemblyandChiasma FormationinCaenorhabditiselegansMeiosis SaritSmolikov,KristinaSchild-Pru¨fert,Mo′nicaP.Colaia′covo* DepartmentofGenetics,HarvardMedicalSchool,Boston,Massachusetts,UnitedStatesofAmerica Abstract The proper assembly of the synaptonemal complex (SC) between homologs is critical to ensure accurate meiotic chromosome segregation. The SC is a meiotic tripartite structure present from yeast to humans, comprised of proteins assembledalongtheaxesofthechromosomesandcentralregion(CR)proteinsthatbridgethetwochromosomeaxes.Here weidentifySYP-4asanovelstructuralcomponentoftheSCinCaenorhabditiselegans.SYP-4interactsinayeasttwo-hybrid assay with SYP-3, one of components of the CR of the SC, and is localized at the interface between homologs during meiosis.SYP-4isessentialforthelocalizationofSYP-1,SYP-2,andSYP-3CRproteinsontochromosomes,therebyplayinga crucial role in the stabilization of pairing interactions between homologous chromosomes. In the absence of SYP-4, the levels of recombination intermediates, as indicated by RAD-51 foci, are elevated in mid-prophase nuclei, and crossover recombination events are significantly reduced. The lack of chiasmata observed in syp-4 mutants supports the elevated levels of chromosome nondisjunction manifested in high embryonic lethality. Altogether our findings place SYP-4 as a centralplayerinSCformationandbroadenourunderstandingofthestructureoftheSCanditsassembly. Citation: Smolikov S, Schild-Pru¨fert K, Colaia′covo MP (2009) A Yeast Two-Hybrid Screen for SYP-3 Interactors Identifies SYP-4, a Component Required for SynaptonemalComplexAssemblyandChiasmaFormationinCaenorhabditiselegansMeiosis.PLoSGenet5(10):e1000669.doi:10.1371/journal.pgen.1000669 Editor:MathildeGrelon,InstitutJean-PierreBourgin,INRAdeVersailles,France ReceivedApril24,2009;AcceptedSeptember1,2009;PublishedOctober2,2009 Copyright: ? 2009 Smolikov et al. This is