Abscisic Acid Refines the Synthesis of Chloroplast Proteins in Maize (Zea mays) in Response to Drought and Light 英文参考文献.docVIP

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Abscisic Acid Refines the Synthesis of Chloroplast Proteins in Maize (Zea mays) in Response to Drought and Light 英文参考文献.doc

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Abscisic Acid Refines the Synthesis of Chloroplast Proteins in Maize (Zea mays) in Response to Drought and Light 英文参考文献

AbscisicAcidRefinestheSynthesisofChloroplast ProteinsinMaize(Zeamays)inResponsetoDroughtand Light XiuliHu1,2,XiaolinWu2,ChaohaiLi1,3,MinghuiLu2,TianxueLiu1,3,YingWang2,WeiWang1,2* 1KeyLaboratoryofPhysiologicalEcologyandGeneticImprovementofFoodCropsinHenanProvince,HenanAgriculturalUniversity,Zhengzhou,China,2CollegeofLife Science,HenanAgriculturalUniversity,Zhengzhou,China,3CollegeofAgronomy,HenanAgriculturalUniversity,Zhengzhou,China Abstract To better understand abscisic acid (ABA) regulation of the synthesis of chloroplast proteins in maize (Zea mays L.) in response to drought and light, we compared leaf proteome differences between maize ABA-deficient mutant vp5 and correspondingwild-typeVp5greenandetiolatedseedlingsexposedtodroughtstress.Proteinsextractedfromtheleavesof Vp5 and vp5 seedlings were used for two-dimensional electrophoresis (2-DE) and subsequent matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). After Coomassie brilliant blue staining, approximately 450 protein spots were reproducibly detected on 2-DE gels. A total of 36 differentially expressed protein spots in response to drought and light were identified using MALDI-TOF MS and their subcellular localization was determinedbasedontheannotationofreviewedaccessioninUniProtKnowledgebaseandthesoftwareprediction.Asa result,corresponding13proteinsofthe24differentiallyexpressedproteinspotsweredefinitelylocalizedinchloroplasts andtheirexpressionwasinanABA-dependentway,including6up-regulatedbybothdroughtandlight,5up-regulatedby droughtbutdown-regulatedbylight,5up-regulatedbylightbutdown-regulatedbydrought;5proteinsdown-regulated by drought were mainly those involved in photosynthesis and ATP synthesis. Thus, the results in the present study supportedthevitalroleofABAinregulatingthesynthesisofdrought-and/orlight-inducedproteinsinmaizechloroplasts andwouldfacilitatethefunctionalcharacterizationofABA-inducedchloroplastproteinsinC4plants. Citation:HuX,WuX,LiC,LuM,