Acidithiobacillus caldus Sulfur Oxidation Model Based on Transcriptome Analysis between the Wild Type and Sulfur Oxygenase Reductase Defective Mutant 英文参考文献.docVIP

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Acidithiobacillus caldus Sulfur Oxidation Model Based on Transcriptome Analysis between the Wild Type and Sulfur Oxygenase Reductase Defective Mutant 英文参考文献.doc

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Acidithiobacillus caldus Sulfur Oxidation Model Based on Transcriptome Analysis between the Wild Type and Sulfur Oxygenase Reductase Defective Mutant 英文参考文献

AcidithiobacilluscaldusSulfurOxidationModelBasedon TranscriptomeAnalysisbetweentheWildTypeand SulfurOxygenaseReductaseDefectiveMutant LinxuChen1,YilinRen2,JianqunLin1*,XiangmeiLiu1,XinPang1,JianqiangLin1 1StateKeyLabofMicrobialTechnology,ShandongUniversity,Jinan,China,2SchoolofLifeScience,ShandongNormalUniversity,Jinan,China Abstract Background:Acidithiobacilluscaldus(A.caldus)iswidelyusedinbio-leaching.Itgainsenergyandelectronsfromoxidation of elemental sulfur and reduced inorganic sulfur compounds (RISCs) for carbon dioxide fixation and growth. Genomic analysessuggestthatitssulfuroxidationsysteminvolvesatruncatedsulfuroxidation(Sox)system(omittingSoxCD),non- Sox sulfur oxidation system similar to the sulfur oxidation in A. ferrooxidans, and sulfur oxygenase reductase (SOR). The complexity of the sulfur oxidation system of A. caldus generates a big obstacle on the research of its sulfur oxidation mechanism. However, thedevelopment of geneticmanipulation methodfor A. caldus in recentyears providespowerful toolsforconstructinggeneticmutantstostudythesulfuroxidationsystem. Results:AnA.caldusmutantlackingthesulfuroxygenasereductasegene(sor)wascreatedanditsgrowthabilitieswere measuredinmediausingelementalsulfur(S0)andtetrathionate(K2S4O6)asthesubstrates,respectively.Then,comparative 0 transcriptomeanalysis(microarraysandreal-timequantitativePCR)ofthewildtypeandtheDsormutantinS andK2S4O6 media were employed to detect the differentially expressed genes involved in sulfur oxidation. SOR was concluded to oxidize the cytoplasmic elemental sulfur, but could not couple the sulfur oxidation with the electron transfer chain or substrate-level phosphorylation. Other elemental sulfur oxidation pathways including sulfur diooxygenase (SDO) and heterodisulfide reductase (HDR), the truncated Sox pathway, and the S4I pathway for hydrolysis of tetrathionate and oxidationofthiosulfateinA.caldusareproposedaccordingtoexpressionpatternsofsulfuroxidationgenesandgrowth abilitiesofth