Adaptation of the Black Yeast Wangiella dermatitidis to Ionizing Radiation Molecular and Cellular Mechanisms 英文参考文献.docVIP

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Adaptation of the Black Yeast Wangiella dermatitidis to Ionizing Radiation Molecular and Cellular Mechanisms 英文参考文献.doc

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Adaptation of the Black Yeast Wangiella dermatitidis to Ionizing Radiation Molecular and Cellular Mechanisms 英文参考文献

AdaptationoftheBlackYeastWangielladermatitidisto IonizingRadiation:MolecularandCellularMechanisms KellyL.Robertson1,AnahitaMostaghim1,4,ChristinaA.Cuomo3,CarissaM.Soto1,NikolaiLebedev1, RobertF.Bailey2,ZhengWang1* 1Center for Bio/MolecularScienceandEngineering, NavalResearchLaboratory,Washington, D.C., United States ofAmerica, 2Health Physics Section,Naval Research Laboratory, Washington, D.C., United States of America, 3The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, United States of America, 4College of Science,GeorgeMasonUniversity,Fairfax,Virginia,UnitedStatesofAmerica Abstract Observations of enhanced growth of melanized fungi under low-dose ionizing radiation in the laboratory and in the damaged Chernobyl nuclear reactor suggest they have adapted the ability to survive or even benefit from exposure to ionizingradiation.However,thecellularandmolecularmechanismof fungalresponses tosuchradiationremainspoorly understood.UsingtheblackyeastWangielladermatitidisasamodel,weconfirmedthationizingradiationenhancedcell growthbyincreasingcelldivisionandcellsize.UsingRNA-seqtechnology,wecomparedthetranscriptomicprofilesofthe wildtypeandthemelanin-deficientwdpks1mutantunderirradiationandnon-irradiationconditions.Itwasfoundthatmore than3000genesweredifferentiallyexpressedwhenthesetwostrainswereconstantlyexposedtoalowdoseofionizing radiationandthathalfwereregulatedatleasttwofoldineitherdirection.Functionalanalysisindicatedthatmanygenesfor aminoacidandcarbohydratemetabolismandcellcycleprogressionweredown-regulatedandthatanumberofantioxidant genes and genes affecting membrane fluidity were up-regulated in both irradiated strains. However, the expression of ribosomalbiogenesisgeneswassignificantlyup-regulatedintheirradiatedwild-typestrainbutnotintheirradiatedwdpks1 mutant, implying that melanin might help to contribute radiation energy for protein translation. Furthermore, we demonstratedthatlong-termexposuretolowdosesofradiationsignificantlyincreasedsur