Adaptive Response to DNA-Damaging Agents in Natural Saccharomyces cerevisiae Populations from “Evolution Canyon”, Mt. Carmel, Israel 英文参考文献.docVIP

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Adaptive Response to DNA-Damaging Agents in Natural Saccharomyces cerevisiae Populations from “Evolution Canyon”, Mt. Carmel, Israel 英文参考文献.doc

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Adaptive Response to DNA-Damaging Agents in Natural Saccharomyces cerevisiae Populations from “Evolution Canyon”, Mt. Carmel, Israel 英文参考文献

AdaptiveResponsetoDNA-DamagingAgentsinNatural SaccharomycescerevisiaePopulationsfrom‘‘Evolution Canyon’’,Mt.Carmel,Israel GabrielA.Lidzbarsky,TamarShkolnik,EviatarNevo* InstituteofEvolution,DepartmentofEvolutionaryandEnvironmentalBiology,FacultyofScienceandScienceEducation,UniversityofHaifa,Haifa,Israel Abstract Background:Naturalpopulationsofmostorganisms,especiallyunicellularmicroorganisms,areconstantlyexposedtoharsh environmental factors which affect their growth. UV radiation is one of the most important physical parameters which influencesyeastgrowthinnature.Hereweused46naturalstrainsofSaccharomycescerevisiaeisolatedfromseveralnatural populationsatthe‘‘EvolutionCanyon’’microsite(NahalOren,Mt.Carmel,Israel).Theopposingslopesofthiscanyonshare the same geology, soil, and macroclimate, but they differ in microclimatic conditions. The interslope differences in solar radiation(200%–800%moreonthe‘‘African’’slope)causedthedevelopmentoftwodistinctbiomes.Thesouth-facingslope is sunnier and has xeric, savannoid ‘‘African’’ environment while the north-facing slope is represented by temperate, ‘‘European’’forestedenvironment.HerewestudiedthephenotypicresponseoftheS.cerevisiaestrainstoUVAandUVC radiations and to methyl methanesulfonate (MMS) in order to evaluate the interslope effect on the strains’ ability to withstandDNA-damagingagents. Methodology/PrincipalFindings:WeexposedourstrainstothedifferentDNA-damagingagentsandmeasuredsurvivalby countingcolonyformingunits.Thestrainsfromthe‘‘African’’slopeweremoreresilienttobothUVAandMMSthanthe strainsfromthe‘‘European’’slope.Incontrast,wefoundthattherewasalmostnodifferencebetweenstrains(withsimilar ploidy)fromtheoppositeslopes,intheirsensitivitytoUVCradiation.Theseresultssuggestthatthe‘‘African’’strainsare moreadaptedtohighersolarradiationthanthe‘‘European’’strains.Wealsofoundthatthetetraploidsstrainsweremore toleranttoallDNA-damagingagentsthantheirneighboringdiploidstrains,whichsuggestthathighploidylevelmightbea mechanismofadap