- 1、本文档共9页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Adenosine Triphosphate Stimulates Aquifex aeolicus MutL Endonuclease Activity 英文参考文献
AdenosineTriphosphateStimulatesAquifexaeolicus
MutLEndonucleaseActivity
JeromeMauris,ThomasC.Evans,Jr*
NewEnglandBiolabs,Inc.,Ipswich,Massachusetts,UnitedStatesofAmerica
Abstract
Background: Human PMS2 (hPMS2) homologues act to nick 59 and 39 to misincorporated nucleotides during mismatch
repairinorganismsthatlackMutH.Mn waspreviouslyfoundtostimulatetheendonucleaseactivityofthesehomologues.
++
ATPwasrequiredforthenickingactivityofhPMS2andyPMS1,butwasreportedtoinhibitbacterialMutLproteinsfrom
Thermus thermophilus and Aquifex aeolicus that displayed homology to hPMS2. Mutational analysis has identified the
DQHA(X)2E(X)4EmotifpresentintheC-terminusofPMS2homologuesasimportantforendonucleaseactivity.
Methodologies/PrincipalFindings:WeexaminedtheeffectATPhadontheMn++inducednickingofsupercoiledpBR322
byfull-lengthandmutantA.aeolicusMutL(AaeMutL)proteins.Assaysweresingletimepoint,enzymetitrationexperiments
or reaction time courses. The maximum velocity for MutL nicking was determined to be 1.660.0861025s21
and
intheabsenceandpresenceofATP,respectively.AMPPNPstimulatedthenickingactivitytoasimilar
4.260.3610
25 21
s
extentasATP.AtruncatedAaeMutLproteincomposedofonlytheC-terminal123aminoacidresidueswasfoundtonick
supercoiledDNA.Furthermore,mutationsintheconservedC-terminalDQHA(X)2E(X)4EandCPHGRPmotifswereshownto
abolishAaeMutLendonucleaseactivity.
Conclusions:ATPstimulatedtheMn++inducedendonucleaseactivityofAaeMutL.ExperimentsutilizingAMPPNPimplied
thatthestimulationdidnotrequireATPhydrolysis.AmutationintheDQHA(X)2E(X)4EmotifofAaeMutLfurthersupported
the role of this region in endonclease activity. For the first time, to our knowledge, we demonstrate that changing the
histidineresidueintheconservedCPHGRPmotifabolishesendonucleolyticactivityofahPMS2homologue.Finally,theC-
++
terminal123aminoacidresiduesofAaeMutLweresufficienttodisplayMn inducednickingactivity.
Citation: Mauris J, Evans TC Jr (2009) Adenosine Triphosphate Stimulates Aquifex aeolicus MutL Endonucleas
您可能关注的文档
- Acute Multiple Organ Failure in Adult Mice Deleted for the Developmental Regulator Wt1 英文参考文献.doc
- Acyclic Identification of Aptamers for Human alpha-Thrombin Using Over-Represented Libraries and Deep Sequencing 英文参考文献.doc
- Acyloxy Nitroso Compounds Inhibit LIF Signaling in Endothelial Cells and Cardiac Myocytes Evidence That STAT3 Signaling Is Redox-Sensitive 英文参考文献.doc
- Acyl Chains of Phospholipase D Transphosphatidylation Products in Arabidopsis Cells A Study Using Multiple Reaction Monitoring Mass Spectrometry 英文参考文献.doc
- Acute Vhl Gene Inactivation Induces Cardiac HIF-Dependent Erythropoietin Gene Expression 英文参考文献.doc
- Adaptation for Protein Synthesis Efficiency in a Naturally Occurring Self-Regulating Operon 英文参考文献.doc
- ADAM10 Releases a Soluble Form of the GPNMBOsteoactivin Extracellular Domain with Angiogenic Properties 英文参考文献.doc
- Adaptation and Immunity 英文参考文献.doc
- Adaptation of High-Growth Influenza H5N1 Vaccine Virus in Vero Cells Implications for Pandemic Preparedness 英文参考文献.doc
- Acylpeptide Hydrolase Inhibition as Targeted Strategy to Induce Proteasomal Down-Regulation 英文参考文献.doc
文档评论(0)