Adaptation of High-Growth Influenza H5N1 Vaccine Virus in Vero Cells Implications for Pandemic Preparedness 英文参考文献.docVIP

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Adaptation of High-Growth Influenza H5N1 Vaccine Virus in Vero Cells Implications for Pandemic Preparedness 英文参考文献.doc

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Adaptation of High-Growth Influenza H5N1 Vaccine Virus in Vero Cells Implications for Pandemic Preparedness 英文参考文献

AdaptationofHigh-GrowthInfluenzaH5N1Vaccine VirusinVeroCells:ImplicationsforPandemic Preparedness Yu-FenTseng.,AlanYung-ChihHu.,Mei-LiangHuang,Wei-ZhouYeh,Tsai-ChuanWeng,Yu-Shuan Chen¤,PeleChong,Min-ShiLee* NationalInstituteofInfectiousDiseasesandVaccinology,NationalHealthResearchInstitutes,Miaoli,Taiwan Abstract Currentegg-basedinfluenzavaccineproductiontechnologycan’tpromptlymeettheglobaldemandduringaninfluenza pandemic as shown in the 2009 H1N1 pandemic. Moreover, its manufacturing capacity would be vulnerable during pandemics caused by highly pathogenic avian influenza viruses. Therefore, vaccine production using mammalian cell technology is becoming attractive. Current influenza H5N1 vaccine strain (NIBRG-14), a reassortant virus between A/ Vietnam/1194/2004(H5N1)virusandegg-adaptedhigh-growthA/PR/8/1934virus,couldgrowefficientlyineggsandMDCK cells but not Vero cells which is the mostpopular cellline formanufacturing human vaccines. Afterserial passages and plaquepurificationsoftheNIBRG-14vaccinevirusinVerocells,onehigh-growthvirusstrain(Vero-15)wasgeneratedand cangrowover108TCID50/ml.Inconclusion,onehigh-growthH5N1vaccineviruswasgeneratedinVerocells,whichcanbe usedtomanufactureinfluenzaH5N1vaccinesandpreparereassortantvaccinevirusesforotherinfluenzaAsubtypes. Citation:TsengY-F,HuAY-C,HuangM-L,YehW-Z,WengT-C,etal.(2011)AdaptationofHigh-GrowthInfluenzaH5N1VaccineVirusinVeroCells:Implicationsfor PandemicPreparedness.PLoSONE6(10):e24057.doi:10.1371/journal.pone.0024057 Editor:AndrewPekosz,JohnsHopkinsUniversity-BloombergSchoolofPublicHealth,UnitedStatesofAmerica ReceivedFebruary21,2011;AcceptedAugust1,2011;PublishedOctober13,2011 Copyright: ? 2011 Tseng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisstudywasfundedbyNationalHealthResearchInstitutes,theTaiwanPandemicInfluenz