Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops 英文参考文献.docVIP

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Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops 英文参考文献.doc

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Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops 英文参考文献

AllergenicityAssessmentofAlliumsativumLeaf Agglutinin,aPotentialCandidateProteinforDeveloping SapSuckingInsectResistantFoodCrops HossainAliMondal1,DipankarChakraborti1,2,PralayMajumder1,PampaRoy3,AmitRoy1,SwatiGupta Bhattacharya3,SampaDas1* 1DivisionofPlantBiology,BoseInstitute,Kolkata,WestBengal,India, 2PostGraduate DepartmentofBiotechnology,St.Xavier’sCollege,Kolkata,WestBengal,India, 3DivisionofPlantBiology,BoseInstitute,Kolkata,WestBengal,India Abstract Background:Mannose-bindingAlliumsativumleafagglutinin(ASAL)ishighlyantinutritionalandtoxictovariousphloem- feedinghemipteraninsects.ASALhasbeenexpressedinanumberofagriculturallyimportantcropstodevelopresistance againstthoseinsects.AwarenessofthesafetyaspectofASALisabsolutelyessentialfordevelopingASALtransgenicplants. Methodology/PrincipalFindings:FollowingtheguidelinesframedbytheFoodandAgricultureOrganization/WorldHealth Organization,thesourceofthegene,itssequencehomologywithpotentallergens,clinicaltestsonmammaliansystems, andthepepsinresistanceandthermostabilityoftheproteinwereconsideredtoaddresstheissue.Nosignificanthomology totheASALsequencewasdetectedwhencomparedtoknownallergenicproteins.TheELISAofbloodseracollectedfrom knownallergypatientsalsofailedtoshowsignificantevidenceofcross-reactivity.Invitroandinvivoassaysbothindicated thedigestibilityofASALinthepresenceofpepsininaminimumtimeperiod. Conclusions/Significance:Withtheseexperiments,weconcludedthatASALdoesnotpossessanyapparentfeaturesofan allergen.Thisisthefirstreportregardingthemonitoringoftheallergenicityofanymannose-bindingmonocotlectinhaving insecticidalefficacyagainsthemipteraninsects. Citation:MondalHA,ChakrabortiD,MajumderP,RoyP,RoyA,etal.(2011)AllergenicityAssessmentofAlliumsativumLeafAgglutinin,aPotentialCandidate ProteinforDevelopingSapSuckingInsectResistantFoodCrops.PLoSONE6(11):e27716.doi:10.1371/journal.pone.0027716 Editor:GuySmagghe,GhentUniversity,Belgium ReceivedJuly29,2011;AcceptedOctober22,2011;PublishedNovember16,2011 Copyright: