Allogeneic Non-Adherent Bone Marrow Cells Facilitate Hematopoietic Recovery but Do Not Lead to Allogeneic Engraftment 英文参考文献.docVIP

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Allogeneic Non-Adherent Bone Marrow Cells Facilitate Hematopoietic Recovery but Do Not Lead to Allogeneic Engraftment 英文参考文献.doc

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Allogeneic Non-Adherent Bone Marrow Cells Facilitate Hematopoietic Recovery but Do Not Lead to Allogeneic Engraftment 英文参考文献

AllogeneicNon-AdherentBoneMarrowCellsFacilitate HematopoieticRecoverybutDoNotLeadtoAllogeneic Engraftment StephanFricke1,2*,ManuelaAckermann3,AlexandraStolzing1,ChristophSchimmelpfennig1 ,Nadja Hilger1,JuttaJahns4,GuidoHildebrandt5,FrankEmmrich1,2,3,PeterRuschpler1,ClaudiaPo¨sel1 ,Manja Kamprad1,2.,UlrichSack1,2,3. 1FraunhoferInstituteforCellTherapyandImmunology(IZI),Leipzig,Germany,2InstituteofClinicalImmunologyandTransfusionMedicine,UniversityofLeipzig,Leipzig, Germany,3TranslationalCentreforRegenerativeMedicine,UniversityofLeipzig,Leipzig,Germany,4ClinicforRadiationTherapyandRadiationOncology,Universityof Leipzig,Leipzig,Germany,5ClinicforRadiationTherapy,UniversityofRostock,Rostock,Germany Abstract Background: Non adherent bone marrow derived cells (NA-BMCs) have recently been described to give rise to multiple mesenchymalphenotypesandhaveanimpactintissueregeneration.Therefore,theeffectsofmurinebonemarrowderived NA-BMCs were investigated with regard to engraftment capacities in allogeneic and syngeneic stem cell transplantation usingtransgenic,humanCD4 ,murineCD42/2,HLA-DR3+mice. + Methodology/PrincipalFindings:BonemarrowcellswereharvestedfromC57Bl/6andBalb/cwild-typemice,expandedto NA-BMCs for 4 days and characterized by flow cytometry before transplantation in lethally irradiated recipient mice. ChimerismwasdetectedusingflowcytometryforMHC-I(H-2D[b],H-2K[d]),mu/huCD4,andhuHLA-DR3).Culturingofbone marrowcellsinadexamethasonecontainingDMEMmediuminducedexpansionofnonadherentcellsexpressingCD11b, CD45,andCD90.AnalysisoftheCD45 showeddepletionofCD4 ,CD8 ,CD19 ,andCD117+cells.Expandedsyngeneicand + + + + allogeneicNA-BMCsweretransplantedintotripletransgenicmice.SyngeneicNA-BMCsprotected83%ofmicefromdeath (n=8, CD4 donor chimerism of 5.862.4% [day 40], P,.001). Allogeneic NA-BMCs preserved 62.5% (n=8) of mice from + death without detectable hematopoietic donor chimerism. Transplantation of syngeneic bone marrow cells preserved 100%,transplantationofallogeneicb