An Integrated Genetic and Cytogenetic Map of the Cucumber Genome 英文参考文献.docVIP

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An Integrated Genetic and Cytogenetic Map of the Cucumber Genome 英文参考文献.doc

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An Integrated Genetic and Cytogenetic Map of the Cucumber Genome 英文参考文献

AnIntegratedGeneticandCytogeneticMapofthe CucumberGenome YiRen1.,ZhonghuaZhang1.,JinhuaLiu2.,JackE.Staub3,YonghuaHan2,ZhouchaoCheng1 ,Xuefeng Li1,JingyuanLu2,HanMiao1,HouxiangKang1,BingyanXie1,XingfangGu1,XiaowuWang1 ,Yongchen Du1,WeiweiJin2*,SanwenHuang1* 1InstituteofVegetablesandFlowers,ChineseAcademyofAgriculturalSciences,Sino-DutchJointLabofHorticulturalGenomics,OpeningLabofGeneticImprovementof AgriculturalCropsofMinistryofAgriculture,Beijing,China,2NationalMaizeImprovementCenterofChina,KeyLaboratoryofCropGeneticImprovementandGenomeof MinistryofAgriculture,BeijingKeyLaboratoryofCropGeneticImprovement,ChinaAgriculturalUniversity,Beijing,China,3USDA,ARS,VegetableCropsResearchUnit, DepartmentofHorticulture,UniversityofWisconsin,Madison,Wisconsin,UnitedStatesofAmerica Abstract TheCucurbitaceaeincludesimportantcropssuchascucumber,melon,watermelon,squashandpumpkin.However,few genetic and genomic resources are available for plant improvement. Some cucurbit species such as cucumber have a narrowgeneticbase,whichimpedesconstructionofsaturatedmolecularlinkagemaps.Wereporthereinthedevelopment ofhighlypolymorphicsimplesequencerepeat(SSR)markersoriginatedfromwholegenomeshotgunsequencingandthe subsequentconstructionofahigh-densitygeneticlinkagemap.Thismapincludes995SSRsinsevenlinkagegroupswhich spans in total 573cM, and defines ,680 recombination breakpoints with an average of 0.58cM between two markers. These linkage groups were then assigned to seven corresponding chromosomes using fluorescent in situ hybridization (FISH).FISHassaysalsorevealedachromosomalinversionbetweenCucumissubspecies[C.sativusvar.sativusL.andvar. hardwickii (R.) Alef], which resulted in marker clustering on the genetic map. A quarter of the mapped markers showed relativelyhighpolymorphismlevelsamong11inbredlinesofcucumber.Amongthe995markers,49%,26%and22%were conservedinmelon,watermelonandpumpkin,respectively.Thismapwillfacilitatewholegenomesequencing,positional cloning,andmolecularbreedingincucumber,a