An NF-Y-Dependent Switch of Positive and Negative Histone Methyl Marks on CCAAT Promoters 英文参考文献.docVIP

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An NF-Y-Dependent Switch of Positive and Negative Histone Methyl Marks on CCAAT Promoters 英文参考文献.doc

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An NF-Y-Dependent Switch of Positive and Negative Histone Methyl Marks on CCAAT Promoters 英文参考文献

AnNF-Y-DependentSwitchofPositiveandNegative HistoneMethylMarksonCCAATPromoters GiacomoDonati.,RaffaellaGatta.,DilettaDolfini,AndreaFossati,MicheleCeribelli,Roberto Mantovani* DipartimentodiScienzeBiomolecolarieBiotecnologie,Universita` diMilano,Milano,Italy Abstract Background: Histone tails have a plethora of different post-translational modifications, which are located differently in ‘‘open’’and‘‘closed’’partsofgenomes.H3K4me3/H3K79me2andH4K20me3areamongthehistonemarksassociatedwith theearlyestablishmentofactiveandinactivechromatin,respectively.Oneofthemostwidespreadpromoterelementsis theCCAATbox,boundbytheNF-Ytrimer.TwoofNF-YsubunitshaveanH2A-H2B-likestructure. Principalfindings:WeestablishedthecausalrelationshipbetweenNF-Ybindingandpositioningofmethylmarks,byChIP analysisofmouseandhumancellsinfectedwithadominantnegativeNF-YA:aparalleldecreaseinNF-Ybinding,H3K4me3, H3K79me2andtranscriptionwasobservedinpromotersthataredependentuponNF-Y.Onthecontrary,changesinthe levelsofH3K9-14acweremoresubtle.ComponentsoftheH3K4methylatingMLLcomplexarenotrecruitedintheabsence ofNF-Y.Asforrepressedpromoters,NF-YremovalleadstoadecreaseintheH4K20me3markanddepositionofH3K4me3. Conclusions: Two relevant findings are reported: (i) NF-Y gains access to its genomic locations independently from the presence of methyl histone marks, either positive or negative; (ii) NF-Y binding has profound positive or negative consequencesonthedepositionofhistonemethylmarks.ThereforeNF-Yisafundamentalswitchattheheartofdecision betweengeneactivationandrepressioninCCAATregulatedgenes. Citation:DonatiG,GattaR,DolfiniD,FossatiA,CeribelliM,etal.(2008)AnNF-Y-DependentSwitchofPositiveandNegativeHistoneMethylMarksonCCAAT Promoters.PLoSONE3(4):e2066.doi:10.1371/journal.pone.0002066 Editor:AxelImhof,UniversityofMunichandCenterofIntegratedProteinScience,Germany ReceivedFebruary7,2008;AcceptedMarch11,2008;PublishedApril30,2008 Copyright: ? 2008 Donati et al. This is an open-access article distributed under the