- 1、本文档共10页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Analysis of Sequence Conservation at Nucleotide Resolution 英文参考文献
AnalysisofSequenceConservation
atNucleotideResolution
Saurabh Asthana1,Mikhail Roytberg2,John Stamatoyannopoulos3*,Shamil Sunyaev1*
1DivisionofGenetics,BrighamandWomen’sHospital,HarvardMedicalSchool,Boston,Massachusetts,UnitedStatesofAmerica,2ComputationalBiologyGroup,Institute
ofMathematicalProblemsinBiology,RussianAcademyofSciences,Pushchino,Russia,3DepartmentofGenomeSciences,UniversityofWashington,Seattle,Washington,
UnitedStatesofAmerica
Oneofthemajorgoalsofcomparativegenomicsistounderstandtheevolutionaryhistoryofeachnucleotideinthe
humangenomesequence,andthedegreetowhichitisunderselectivepressure.Ascertainmentofselectiveconstraint
atnucleotideresolutionisparticularlyimportantforpredictingthefunctionalsignificanceofhumangeneticvariation
and for analyzing the sequence substructure of cis-regulatory sequences and other functional elements. Current
methods for analysis of sequence conservation are focused on delineation of conserved regions comprising tens or
even hundreds of consecutive nucleotides. We therefore developed a novel computational approach designed
specificallyforscoringevolutionaryconservationatindividualbase-pairresolution.Ourapproachestimatestherateat
which each nucleotide position is evolving, computes the probability of neutrality given this rate estimate, and
summarizes the result in a Sequence CONservation Evaluation (SCONE) score. We computed SCONE scores in a
continuous fashion across 1% of the human genome for which high-quality sequence information from up to 23
genomes are available. We show that SCONE scores are clearly correlated with the allele frequency of human
polymorphismsinbothcodingandnoncodingregions.Wefindthatthemajorityofnoncodingconservednucleotides
lie outside of longer conserved elements predicted by other conservation analyses, and are experiencing ongoing
selectioninmodernhumansasevidentfromtheallelefrequencyspectrumofhumanpolymorphism.Wealsoapplied
SCONE to analyze the distribution of conserved nucleotides within funct
您可能关注的文档
- An “Escape Clock” for Estimating the Turnover of SIV DNA in Resting CD4+ T Cells 英文参考文献.doc
- Analysis of C3 Suggests Three Periods of Positive Selection Events and Different Evolutionary Patterns between Fish and Mammals 英文参考文献.doc
- Analysis of Amino Acid Variation in the P2 Domain of the GII-4 Norovirus VP1 Protein Reveals Putative Variant-Specific Epitopes 英文参考文献.doc
- Analysis of 16S rRNA Amplicon Sequencing Options on the Roche454 Next-Generation Titanium Sequencing Platform 英文参考文献.doc
- Analysis of Cancer Mutation Signatures in Blood by a Novel Ultra-Sensitive Assay Monitoring of Therapy or Recurrence in Non-Metastatic Breast Cancer 英文参考文献.doc
- Analysis of an Ordered, Comprehensive STM Mutant Library in Infectious Borrelia burgdorferi Insights into the Genes Required for Mouse Infectivity 英文参考文献.doc
- Analysis of Candidate Colitis Genes in the Gdac1 Locus of Mice Deficient in Glutathione Peroxidase-1 and -2 英文参考文献.doc
- Analysis of Complete Nucleotide Sequences of 12 Gossypium Chloroplast Genomes Origin and Evolution of Allotetraploids 英文参考文献.doc
- Analysis of Clonal Type-Specific Antibody Reactions in Toxoplasma gondii Seropositive Humans from Germany by Peptide-Microarray 英文参考文献.doc
- Analysis of Combinatorial Regulation Scaling of Partnerships between Regulators with the Number of Governed Targets 英文参考文献.doc
文档评论(0)