Angiogenesis-Related Gene Expression Profile with Independent Prognostic Value in Advanced Ovarian Carcinoma 英文参考文献.docVIP

Angiogenesis-RelatedGeneExpressionProfilewith IndependentPrognosticValueinAdvancedOvarian Carcinoma MartaMendiola1,4.,JorgeBarriuso2,4.,Andre′sRedondo2,Adria′nMarin?o-Enr?′quez1,RosarioMadero3,4, EnriqueEspinosa2,JuanAngel′ FresnoVara4,IkerSa′nchez-Navarro4,Gine′sHerna′ndez-Cortes5 ,Pilar Zamora2,EliaPe′rez-Ferna′ndez3,4,Mar?′aMiguel-Mart?′n1,4,Asuncio′nSua′rez1,Jose′ Palacios5 ,Manuel Gonza′lez-Baro′n2,DavidHardisson1* 1DepartmentofPathology,HospitalUniversitarioLaPaz,UniversidadAuto′nomadeMadrid,Madrid,Spain,2TranslationalOncologyUnit,HospitalUniversitarioLaPaz, UniversidadAuto′nomadeMadrid,Madrid,Spain,3UnitofBiostatistics,HospitalUniversitarioLaPaz,UniversidadAuto′nomadeMadrid,Madrid,Spain,4Fundacio′npara laInvestigacio′nBiome′dica delHospitalUniversitarioLaPaz(FIBHULP),Madrid,Spain,5DepartmentofPathology,HospitalUniversitarioV?′rgendelRoc?′o,Sevilla,Spain Abstract Background:Ovariancarcinomaisthemostimportantcauseofgynecologicalcancer-relatedmortalityinWesternsocieties. Despite the improved median overall survival in patients receiving chemotherapy regimens such as paclitaxel and carboplatincombination,relapsestilloccursinmostadvanceddiseasedpatients.Increasedangiogenesisisassociatedwith rapidrecurrenceanddecreasedsurvivalinovariancancer.Thisstudywasplannedtoidentifyanangiogenesis-relatedgene expressionprofilewithprognosticvalueinadvancedovariancarcinomapatients. Methodology/PrincipalFindings:RNAswerecollectedfromformalin-fixedparaffin-embeddedsamplesof61patientswith III/IVFIGOstageovariancancerwhounderwentsurgicalcytoreductionandreceivedacarboplatinpluspaclitaxelregimen. Expression levels of 82 angiogenesis related genes were measured by quantitative real-time polymerase chain reaction using TaqMan low-density arrays. A 34-gene-profile which was able to predict the overall survival of ovarian carcinoma patientswasidentified.Afteraleave-one-outcrossvalidation,theprofiledistinguishedtwogroupsofpatientswithdifferent outcomes. Median overall survival