Anx2 Interacts with HIV-1 Gag at Phosphatidylinositol (4,5) Bisphosphate-Containing Lipid Rafts and Increases Viral Production in 293T Cells 英文参考文献.docVIP

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Anx2 Interacts with HIV-1 Gag at Phosphatidylinositol (4,5) Bisphosphate-Containing Lipid Rafts and Increases Viral Production in 293T Cells 英文参考文献.doc

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Anx2 Interacts with HIV-1 Gag at Phosphatidylinositol (4,5) Bisphosphate-Containing Lipid Rafts and Increases Viral Production in 293T Cells 英文参考文献

Anx2InteractswithHIV-1GagatPhosphatidylinositol (4,5)Bisphosphate-ContainingLipidRaftsandIncreases ViralProductionin293TCells AlexiaV.Harrist,ElenaV.Ryzhova,ThomasHarvey,FranciscoGonza′lez-Scarano* DepartmentsofNeurologyandMicrobiology,UniversityofPennsylvania,Philadelphia,Pennsylvania,UnitedStatesofAmerica Abstract The neuronal damage characteristic of HIV-1-mediated CNS diseases is inflicted by HIV-1 infected brain macrophages. Severalstepsofviralreplication,includingassemblyandbudding,differbetweenmacrophagesandTcells;itislikelythat cell-specifichostfactorsmediatethesedifferences.WepreviouslydefinedAnnexin2(Anx2)asanHIVGagbindingpartner in human monocyte-derived macrophages (MDMs) that promotes proper viral assembly. Anx2, a calcium-dependent membrane-binding protein that can aggregate phospholipid-containing lipid rafts, is expressed to high levels in macrophages,butnotinTlymphocytesorthe293Tcellline.Here,weusebimolecularfluorescencecomplementationin the 293T cell model to demonstrate that Anx2 and HIV-1 Gag interact at the phosphatidylinositol (4,5) bisphosphate- containinglipidraftmembranedomainsatwhichGagmediatesviralassembly.Furthermore,wedemonstratethatAnx2 expressionin293TcellsincreasesGagprocessingandHIV-1production.ThesedataprovidenewevidencethatAnx2,by interactingwithGagatthemembranesthatsupportviralassembly,functionsinthelatestagesofHIV-1replication. Citation:HarristAV,RyzhovaEV,HarveyT,Gonza′lez-ScaranoF(2009)Anx2InteractswithHIV-1GagatPhosphatidylinositol(4,5)Bisphosphate-ContainingLipid RaftsandIncreasesViralProductionin293TCells.PLoSONE4(3):e5020.doi:10.1371/journal.pone.0005020 Editor:CherylA.Stoddart,UniversityofCaliforniaSanFrancisco,UnitedStatesofAmerica ReceivedNovember14,2008;AcceptedFebruary24,2009;PublishedMarch27,2009 Copyright: ? 2009 Harrist et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheorig