Arabidopsis R-SNARE Proteins VAMP721 and VAMP722 Are Required for Cell Plate Formation 英文参考文献.docVIP

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Arabidopsis R-SNARE Proteins VAMP721 and VAMP722 Are Required for Cell Plate Formation 英文参考文献.doc

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Arabidopsis R-SNARE Proteins VAMP721 and VAMP722 Are Required for Cell Plate Formation 英文参考文献

ArabidopsisR-SNAREProteinsVAMP721andVAMP722 AreRequiredforCellPlateFormation LiangZhang1,2.,HaiyanZhang1.,PengLiu3,HuaiqingHao1,JingBoJin1,JinxingLin1* 1KeyLaboratoryofPlantMolecularPhysiology,InstituteofBotany,ChineseAcademyofSciences,Beijing,China,2GraduateSchoolofChineseAcademyofSciences, Beijing,China,3StateKeyLaboratoryofPlantGenomics,InstituteofMicrobiology,ChineseAcademyofSciences,Beijing,China Abstract Background:CellplateformationduringplantcytokinesisisfacilitatedbySNAREcomplex-mediatedvesiclefusionatthe cell-divisionplane.However,ourknowledgeregardingR-SNAREcomponentsofmembranefusionmachineryforcellplate formationremainsquitelimited. Methodology/Principal Findings: We report the in vivo function of Arabidopsis VAMP721 and VAMP722, two closely sequence-relatedR-SNAREs,incellplateformation.Doublehomozygousvamp721vamp722mutantseedlingsshowedlethal dwarfphenotypesandwerecharacterizedbyrudimentaryroots,cotyledonsandhypocotyls.Furthermore,cellwallstubs andincompletecytokinesiswerefrequentlyobservedinvamp721vamp722seedlings.Confocalimagesrevealedthatgreen fluorescentprotein-taggedVAMP721andVAMP722werepreferentially localizedtotheexpandingcellplates individing cells. Drug treatments and co-localization analyses demonstrated that punctuate organelles labeled with VAMP721 and VAMP722representedearlyendosomesoverlappedwithVHA-a1-labeledTGN,whichweredistinctfromGolgistacksand prevacuolar compartments. In addition, protein traffic to the plasma membrane, but not to the vacuole, was severely disruptedinvamp721vamp722seedlingsbysubcellularlocalizationofmarkerproteins. Conclusion/Significance:TheseobservationssuggestthatVAMP721andVAMP722areinvolvedinsecretorytraffickingto the plasma membrane via TGN/early endosomal compartment, which contributes substantially to cell plate formation duringplantcytokinesis. Citation:ZhangL,ZhangH,LiuP,HaoH,JinJB,etal.(2011)ArabidopsisR-SNAREProteinsVAMP721andVAMP722AreRequiredforCellPlateFormation.PLoS ONE6(10):e26129.doi:10.1371/