Calycosin Promotes Angiogenesis Involving Estrogen Receptor and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathway in Zebrafish and HUVEC 英文参考文献.docVIP

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Calycosin Promotes Angiogenesis Involving Estrogen Receptor and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathway in Zebrafish and HUVEC 英文参考文献.doc

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Calycosin Promotes Angiogenesis Involving Estrogen Receptor and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathway in Zebrafish and HUVEC 英文参考文献

CalycosinPromotesAngiogenesisInvolvingEstrogen ReceptorandMitogen-ActivatedProteinKinase(MAPK) SignalingPathwayinZebrafishandHUVEC JingYanTang1.,ShangLi1.,ZhenHuaLi1,ZaiJunZhang1,GuangHu1,LoritaChiVengCheang1 ,Deepa Alex1,MaggiePuiManHoi1,YiuWaKwan2,ShunWanChan3,GeorgePakHengLeung4,SimonMing YuenLee1* 1InstituteofChineseMedicalSciences,UniversityofMacau,Macao,China,2SchoolofBiomedicalSciences,FacultyofMedicine,TheChineseUniversityofHongKong, HongKong,China,3StateKeyLaboratoryofChineseMedicineandMolecularPharmacology,DepartmentofAppliedBiologyandChemicalTechnology,TheHongKong PolytechnicUniversity,HongKong,China,4DepartmentofPharmacologyandPharmacy,FacultyofMedicine,TheUniversityofHongKong,HongKong,China Abstract Background: Angiogenesis plays an important role in a wide range of physiological processes, and many diseases are associatedwiththedysregulationofangiogenesis.RadixAstragaliisaChinesemedicinalherbcommonlyusedfortreating cardiovasculardisordersandhasbeenshowntopossessangiogeniceffectinpreviousstudiesbutitsactiveconstituentand underlying mechanism remain unclear. The present study investigates the angiogenic effects of calycosin, a major isoflavonoidisolatedfromRadixAstragali,invitroandinvivo. Methodology:Tg(fli1:EGFP)andTg(fli1:nEGFP)transgeniczebrafishembryosweretreatedwithdifferentconcentrationsof calycosin (10, 30, 100mM) from 72hpf to 96hpf prior morphological observation and angiogenesis phenotypes assessment. Zebrafish embryos were exposed to calycosin (10, 100mM) from 72hpf to 78hpf before gene-expression analysis. The effects of VEGFR tyrosine kinase inhibitor on calycosin-induced angiogenesis were studied using 72hpf Tg(fli1:EGFP)andTg(fli1:nEGFP)zebrafishembryos.Thepro-angiogeniceffectsofcalycosinwerecomparedwithraloxifene andtamoxifenin72hpfTg(fli1:EGFP)zebrafishembryos.Thebindingaffinitiesofcalycosintoestrogenreceptors(ERs)were evaluated by cell-free and cell-based estrogen receptor binding assays. Human umbilical vein endothelial cell cultures