反式茴脑生物降解菌株的筛选及其生物转化.pdfVIP

学兔兔 化 工 进 展 2009年第28卷第1期 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS ·141· 反式茴脑生物降解菌株的筛选及其生物转化 粟桂娇1,2刘雄民 ，朱 萍 ，陈 敏 ，莫祺红 ( 广西大学生命科学与技术学院，广西 南宁 530005； 广西大学化学化工学院，广西 南宁 530004) 摘 要：以广西特色香料资源茴油为碳源，开展了反式茴脑生物降解菌株的筛选_Y-作，并对反式茴脑生物转化过 程进行了初步研究，以期获得的菌株能催化茴油中的主要成分反式茴脑，合成高附加值的天然香料或医药中间体。 通过富集培养的方法，从八角树下土壤中筛选能将反式茴脑转化生成茴香醛或茴香酸的细菌菌株，采用薄层层析 法、分光光度法和HPLC法相结合对转化液进行分析。结果筛选到一株细菌菌株BT-13能以茴油为碳源高产茴香 酸。从形态、生理生化实验和16S rDNA序列分析，初步鉴定细菌菌株BT-13为假单胞菌属(Pseudomonas sp．)。 关键词：茴油；反式茴脑；筛选；假单胞菌；生物转化 中图分类号：TQ 65；Q 93 文献标识码：A 文章编号：1000—6613(2009)01—0141—05 Isolation of trans-anethole degrading bacterial strains and biotransformation of trans．anethole SU Guijiao ，LIU Xiongmin ，ZHU Ping ，CHEN Min ，MO Qihong ( School ofLife Science andTechnology．GuangxiUniversity，Nanning 530005，Guangxi，China； School of Chemistry and Chemical Engineering，Guangxi University，Nanning 530004，Guangxi，China) Abstract：Anise oil is a natural spice in Guangxi．Isolation of trans—anethole degrading bacterial swains and trans．anethole biotransformation process were performed using anise oil as the only carbon source． The target strains were expected to produce high value—added nature spice and medicine intermediate by biotransform ation of trans—anethole—_the main constituent of anise oil．Bacteria were isolated from soil under aniseed n．ees by enrichment culture．Then biotransformation of trans—anethole to anisaldehyde or anisic acid with these strains were carried out by fermentation．The contents of trans—anethole， anisaldehyde and anisic acid in the culture broth