叔戊醇体系酶促大豆油制备生物柴油.pdfVIP

学兔兔 化 工 进 展 ·230· CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 2009年第28卷第2期 叔戊醇体系酶促大豆油制备生物柴油 郑 妍，权 静，朱利民，蒋 波 (东华大学化学化工与生物工程学院，上海 201620) 摘 要：叔戊醇作为反应介质，固定化脂肪酶Novozym435催化大豆油与甲醇的转酯反应制备生物柴油。叔戊醇 消除了反应底物甲醇及反应副产物甘油对酶活的负面影响。定量分析表明，叔戊醇与油脂的体积比为 1，甲醇与 油脂的摩尔比为3，2％脂肪酶，反应体系含水量2％，40℃、180 r／min条件下反应15 h，生物柴油得率可达97％。 在最适条件下反应进行160批次，酶仍保持了较高的活性和良好的稳定性。 关键词：生物柴油；叔戊醇；脂肪酶；稳定性 中图分类号：O 643．32 文献标识码：A 文章编号：1000—6613(2009)02—0230—05 Lipase-catalyzed production of biodiesel from soybean oil in tert-amyl alcohol system ZHENG Yan，QUANJing，ZHULimin，JIANG Bo (Collge of Chemistry，Chemical Engineering and Biotechnology，Donghua University，Shanghm 201620，China) Abstract：Tert．amyl alcohol was used as a reaction medium for Novozym 435 lipase．catalyzed methanolysis of soybean oil for biodiese1 production．With tert-amyl alcohol as reaction medium，bOth the negative effects caused by excessive methanol and by—product glycerol could be eliminated．TIle highest biodiesel yield of 97％was obtmned under the optimum conditions(tert．amyl alcohol／oil volume ratio 1，methanol／oil molar ratio 3，2％Novozym 435 based on the soybean oil weight，temperature 40 ℃，2％water content based on soybean oil，180 r／min and 15 h1．Moreover，there was no evident loss in lipase activity and stability even after being repeatedly used for 1 60 runs and the yield of biodiesel production was more than 95％． Key words：biodiesel；tert—am y】alcohol：lipase；stability 生物柴油是由可再生的动植物油脂与短链醇 因为甲醇对酶的毒性以及副产物甘油吸附在酶的表 (甲醇或乙醇)经转酯化反应制得的脂肪酸酯 (甲 面，很大程度上降低了酶的活性及稳定性【8 ]，致