异养产油单针藻的生长、代谢及脂肪酸组成的变化.pdfVIP

学兔兔 化 工 进 展 2013年第32卷第6期 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 异养产油单针藻的生长、代谢及脂肪酸组成的变化 王 琳，邓 涛，徐军伟，赵 鹏，李 涛，余旭亚 (昆明理工大学生命科学与技术学院，云南 昆明650500) 摘 要：单针藻是制备生物柴油较好的原料。以产油单针藻为对象，研究了单针藻在异养培养中生长、代谢的特 性及脂肪酸组成变化。结果表明，Richards模型能够较好拟合藻细胞的生长与培养时间的关系。建立了微藻细胞 对硝酸盐、磷酸盐的代谢模型，得出藻细胞生物量对硝酸盐、磷酸盐的得率系数分别为5_319g／g、500g／g 生长 过程中脂肪酸组成变化的分析表明，延长藻细胞的培养稳定期可增加c18：1的含量。 关键词：产油微藻；单针藻；异养；动力学；脂肪酸 中图分类号：TQ 645 文献标志码：A 文章编号：1000—6613(2013)06—1377—05 Doh 10．3969~．issn．1000—6613．2013．06．030 Growth,metabolic and fatty acid composition of oleaginous Monoraphidium sp．FXY-I O under heterotrophic conditions WANGLin，DENG Tao，XUJunwei，ZHA0 Peng，LI Tao，YUXuya (College ofLife-Science and Biotechnology，Kunming University of Science and Technology，Kunming 650500， Yunnan，China) Abstract：Monoraphidium sp．FXy-l 0 could be used as an ideal candidate for biodiesel production．The paper mainly focuses on the growth，the metabolism characteristics and the change of fatty acid composition of Monoraphidium sp．FXY 1 0，an oleaginous microalga under heterotrophic condition．It is found that the Richards model can fit the relationship between the growth of the cells and culture time． The models ofnitrate and phosphate metabolism ofMonoraphidium sp．FXY-l0 are established，and cell yield coefficients on nitrate，phosphate are 5．3 19 g／g and 500 g／g，respectively~The variation analysis of the fatty acid profiles in the growth process indicates that the extension of the cultivation stabilization period can increase the content of octadecenoic acid． Key words：oleaginous microalge；Monoraphidium sp．；heterotrophy；kinetics