Development and Characterization of a New TILLING Population of Common Bread Wheat (Triticum aestivum L.) 英文参考文献.docVIP

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Development and Characterization of a New TILLING Population of Common Bread Wheat (Triticum aestivum L.) 英文参考文献.doc

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Development and Characterization of a New TILLING Population of Common Bread Wheat (Triticum aestivum L.) 英文参考文献

DevelopmentandCharacterizationofaNewTILLING PopulationofCommonBreadWheat(Triticumaestivum L.) LiangChen1,LinzhouHuang1,DonghongMin1,AndyPhillips2,ShiqiangWang1,PippaJ.Madgwick2, MartinA.J.Parry2,Yin-GangHu1,3* 1StateKeyLaboratoryofCropStressBiologyinAridAreasandCollegeofAgronomy,NorthwestAgriculturalandForestryUniversity,Yangling,Shaanxi,China,2Centre forCropGeneticImprovement,DepartmentofPlantScience,RothamstedResearch,Harpenden,Herts,UnitedKingdom,3InstituteofWaterSavingAgricultureinArid RegionsofChina,NorthwestAgriculturalandForestryUniversity,Yangling,Shaanxi,China Abstract Mutagenesisisanimportanttoolincrop improvement.However, thehexaploid genomeofwheat(TriticumaestivumL.) presents problems in identifying desirable genetic changes based on phenotypic screening due to gene redundancy. TILLING (Targeting Induced Local Lesions IN Genomes), a powerful reverse genetic strategy that allows the detection of induced point mutations in individuals of the mutagenized populations, can address the major challenge of linking sequenceinformationtothebiologicalfunctionofgenesandcanalsoidentifynovelvariationforcropbreeding.Wheatis especiallywell-suitedforTILLINGduetothehighmutationdensitiestoleratedbypolyploids.However,onlyafewwheat TILLING populations are currentlyavailable in theworld, which is far from satisfyingthe requirement ofresearchers and breeders in different growing environments. In addition, current TILLING screening protocols require costly fluorescence detectionsystems,limitingtheiruse,especiallyindevelopingcountries.WedevelopedanewTILLINGresourcecomprising 2610 M2 mutants in a common wheat cultivar ‘Jinmai 47’. Numerous phenotypes with altered morphological and agronomictraitswereobservedfromtheM2andM3linesinthefield.Tosimplifytheprocedureanddecreasecosts,weuse unlabeledprimersandeithernon-denaturingpolyacrylamidegelsoragarosegelsformutationdetection.Thevalueofthis newresourcewastestedusingPCRwithRAPDandIntron-splicedjunction(ISJ)primers,andalsoTILLINGint