Developmental Regulation of Protein O-GlcNAcylation, O-GlcNAc Transferase, and O-GlcNAcase in Mammalian Brain 英文参考文献.docVIP

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Developmental Regulation of Protein O-GlcNAcylation, O-GlcNAc Transferase, and O-GlcNAcase in Mammalian Brain 英文参考文献.doc

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Developmental Regulation of Protein O-GlcNAcylation, O-GlcNAc Transferase, and O-GlcNAcase in Mammalian Brain 英文参考文献

DevelopmentalRegulationofProteinO-GlcNAcylation, O-GlcNAcTransferase,andO-GlcNAcaseinMammalian Brain YingLiu1.¤a,XiaojingLi1.,YangYu1¤b,JianhuaShi1,2,ZhihouLiang1¤c,XiaoqinRun1¤c,YiLi1¤d ,Chun- lingDai1,IngeGrundke-Iqbal1,KhalidIqbal1,FeiLiu1,2,Cheng-XinGong1* 1DepartmentofNeurochemistry,NewYorkStateInstituteforBasicResearchinDevelopmentalDisabilities,StatenIsland,NewYork,UnitedStatesofAmerica,2Jiangsu KeyLaboratoryofNeuroregeneration,NantongUniversity,Nantong,Jiangsu,China Abstract O-GlcNAcylation is a common posttranslational modification of nucleocytoplasmic proteins by b-N-acetylglucosamine (GlcNAc). The dynamic addition and removal of O-GlcNAc groups to and from proteins are catalyzed by O-linked N- acetylglucosamine transferase (O-GlcNAc transferase, OGT) and b-N-acetylglucosaminidase (O-GlcNAcase, OGA), respec- tively. O-GlcNAcylation often modulates protein phosphorylation and regulates several cellular signaling and functions, especiallyinthebrain.However,itsdevelopmentalregulationisnotwellknown.Here,westudiedproteinO-GlcNAcylation, OGT,andOGAintheratbrainatvariousagesfromembryonicday15totheageof2years.Wefoundagradualdeclineof globalproteinO-GlcNAcylationduringdevelopmentalstagesandadulthood.Thisdeclinecorrelatedpositivelytothetotal proteinphosphorylationatserineresidues,butnotatthreonineresidues.TheexpressionofOGTandOGAisoformswas regulateddifferentlyatvariousages.ImmunohistochemicalstudiesrevealedubiquitousdistributionofO-GlcNAcylationat allages.StrongimmunostainingofO-GlcNAc,OGT,andOGAwasobservedmostlyinneuronalcellbodiesandprocesses, furthersuggestingtheroleofO-GlcNAcmodificationofneuronalproteinsinthebrain.Thesestudiesprovidefundamental knowledge of age-dependent protein modification by O-GlcNAc and will help guide future studies on the role of O- GlcNAcylationinthemammalianbrain. Citation: Liu Y, Li X, Yu Y, Shi J, Liang Z, et al. (2012) Developmental Regulation of Protein O-GlcNAcylation, O-GlcNAc Transferase, and O-GlcNAcase in MammalianBrain.PLoSON